牦牛源牛呼吸道合胞体病毒的分子流行病学调查  

Molecular Epidemiological Investigation of Bovine Respiratory Syncytial Virus in Yaks

作　　者：常益铭 汤承[1] 岳华[1] CHANG Yiming;TANG Cheng;YUE Hua(College of Animal&Veterinary Sciences,Southwest Minzu University,Chengdu 610041,China)

机构地区：[1]西南民族大学畜牧兽医学院,成都610041

出　　处：《畜牧兽医学报》2023年第5期2030-2041,共12页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：“十四五”国家重点研发计划课题(2021YFD1600203);国家农业产业技术体系四川肉牛创新团队专项(SCCXTD-2020-13);四川省高等学校重点实验室——动物医学实验室(2021PTJS34)。

摘　　要：牛呼吸道合胞体病毒(bovine respiratory syncytial virus,BRSV)是引起牛呼吸道疾病综合征(bovine respiratory diseases complex,BRDC)的重要病原之一,本试验旨在调查BRSV是否在青藏高原牦牛中存在及其在患呼吸道疾病的牦牛中的分子流行情况。2021年3月―2022年7月,在四川省甘孜藏族自治州(甘孜州)和阿坝藏族羌族自治州(阿坝州)的10个牦牛场中收集了122份患有呼吸道疾病牦牛的深部鼻腔棉拭子,其中,91份样本来自甘孜州,31份样本来自阿坝州,采用反转录恒温隔绝式PCR(reverse transcription insulated isothermal PCR,RT-iiPCR)方法对临床样本进行了BRSV的检测。结果显示,BRSV的平均阳性率为64.75%,其中,甘孜州的样本阳性率为73.63%,阿坝州的样本阳性率为38.71%;场阳性率为100%。从阳性样本中扩增到了10条完整的G基因序列(均为Ⅲ亚群)和9条完整的F基因序列,与GenBank中所有国外的BRSV毒株相比,牦牛源G和F蛋白与本实验室最近上传的BRSV毒株之间存在多个相同的氨基酸位点突变。此外,还获得了1条牦牛源的Ⅲ亚群BRSV全基因组,与本实验室最近上传的国内肉牛源BRSV毒株(GenBank登录号:OP137030~OP137034)遗传关系最近。本研究首次证实了BRSV在牦牛中的存在与流行,获得了1条牦牛源的BRSVⅢ亚群基因组序列,丰富了牦牛的呼吸道疾病的病原谱,为牦牛呼吸道疾病的防控提供了参考。Bovine respiratory syncytial virus(BRSV)is an important pathogen of bovine respiratory disease complex(BRDC).This experiment aims to investigate whether BRSV were present in Tibetan plateau yaks and its molecular prevalence in yaks with respiratory diseases.One hundred and twenty-two nasal swabs from Ganzi(Garzê)Tibetan Autonomous Prefecture and Aba(Ngawa)Tibetan and Qiang Autonomous Prefecture were collected from Yak with BRDC in 10 farms across Sichuan provinces from March 2021 to July 2022,among them,91 samples were from Ganzi Prefecture and 31 samples were from Aba Prefecture.Using reverse transcription insulated isothermal PCR(RT-iiPCR),64.75%of samples tested positive for BRSV.Among them,the positive rate in Ganzi Prefecture and Aba Prefecture was 73.63%and 38.71%respectively,and the farm positive rate was 100%.Further,10 complete G genes were identified as subgroupⅢstrains,and 9 complete F genes were amplified from positive samples.Compared to known BRSV strains in GenBank,G proteins and F proteins from yaks with BRSV strains previously amplified by our laboratory shared several identical amino acid mutations.Moreover,complete genomes from Yaks were obtained,which was closest to the BRSV strain(GenBank accession number:OP137030-OP137034)uploaded recently by our laboratory.In conclusion,this study confirmed the existence and prevalence of BRSV in yaks for the first time,obtained a genome sequence of BRSV in theⅢsubgroup from yaks,enriched the pathogenic spectrum of respiratory diseases in yaks,and provided a reference for the prevention and control of respiratory diseases in yaks.

关 键 词：牦牛 牛呼吸道合胞体病毒 分子流行病学 基因特征 

分 类 号：S852.659.5[农业科学—基础兽医学] S852.653[农业科学—兽医学]