外源物质对红锥生根及其生根过程中相关生理生化特性的影响  

ffEffects of Exogenous Substances on Rooting and Related Physiological and Biochemical Characteristics During Rooting of Castanopsis hystrix

作　　者：温汉平 陈家裕 钟雨庭 钟远芳 郭彦青 WEN Hanping;CHEN Jiayu;ZHONG Yuting;ZHONG Yuanfang;GUO Yanqing(Zijin Ecological Public Welfare Forest Management Center,Heyuan,Guangdong 517400,China;Zijin Forestry Research Institute,Heyuan,Guangdong 517400,China;Guangdong Eco-Engineering Polytechnic,Guangzhou,Guangdong 510520,China)

机构地区：[1]紫金县生态公益林管理中心,广东河源517400 [2]紫金县林业研究所,广东河源517400 [3]广东生态工程职业学院,广东广州510520

出　　处：《林业与环境科学》2023年第2期17-25,共9页Forestry and Environmental Science

摘　　要：以红锥Castanopsis hystrix成年优树嫁接苗萌发新枝为试验材料,通过扦插育苗方法,分析NO供体(SNP)、清除剂(cPTIO)及ABT1处理下插穗生根效果、内源激素、活性氧化物与抗氧化能力的变化。结果表明:供试75~1 000 mg/L SNP、cPTIO、ABT1及对照(清水)16个处理中,以300 mg/L SNP和500~1 000 mg/L ABT1处理下的生根率高,达69.5%~73.6%,但以300 mg/L浓度SNP处理下的根系质量及成活效果最好,平均根条数9.5、根长20.3 mm、成活率99.8%。与其他处理相比,除500~1 000mg/L浓度ABT1处理外,在300 mg/L浓度SNP处理下扦插10~30 d后插穗中内源IAA水平明显偏高,但ZR、GA、ABA水平无显著变化,在扦插30 d生根插穗中可见明显增强的IAA组织化学定位。同时,扦插10 d时在300 mg/L浓度SNP处理下O_(2)·^(-)和H_(2)O_(2)含量、SOD活性显著高于其他任一浓度的cPTIO和ABT1处理。研究认为,相较生产中常用ABT1,NO能有效促进红锥生根能力的提升及根系质量的改善,这与生根过程中NO诱导的IAA水平上升及根原基形成期增加的O_(2)·^(-)、H_(2)O_(2)含量和SOD活性有关。Taking the new branches of grafted seedlings of adult superior trees of Castanopsis hystrix as experimental materials,the effects of NO donor(SNP),scavenger(cPTIO),and ABT1 on rooting,endogenous hormones,active oxides,and antioxidant capacity of cuttings were analyzed.The results showed that among the 16 treatments of 75-1000 mg/L SNP,cPTIO,ABT1,and control(clear water),the rooting rate of 300 mg/L SNP and 500-1000 mg/L ABT1 was 69.5%-73.6%,but the root quality and survival effect of 300 mg/L SNP treatment was the best,with average 9.5 roots,20.3 mm root length and 99.8%survival rate.Compare with other treatments,except 500-1000 mg/L ABT1 treatment,the level of endogenous IAA in cuttings treated with 300 mg/L SNP for 10~30 days was significantly higher,but the consent rations of ZR,GA,and ABA did not change significantly.The content of O_(2)·^(-)and H_(2)O_(2) and SOD activity under 300 mg/L SNP treatment were significantly higher than those under other concentrations of cPTIO and ABT1 treatment.Compared with ABT1 commonly used in production,NO could effectively promote the rooting ability and root quality of C.hystrix,which was related to the increased IAA level induced by NO during rooting and the increased O_(2)·^(-),H_(2)O_(2) content and SOD activity during root primordium formation.

关 键 词：红锥 不定根 信号物质 IAA 活性氧化物 

分 类 号：S722[农业科学—林木遗传育种]