大丽轮枝菌JR2特异的乙醇脱氢酶的生物学功能研究  

Functional characterization of a strain-specific alcohol dehydrogenase in Verticillium dahliae JR2

作　　者：金贤姜 张嘉益 刘廷利 王永林[1] JIN Xianjiang;ZHANG Jiayi;LIU Tingli;WANG Yonglin(Beijing Key Laboratory for Forest Pest Control,Beijing Forestry University,Beijing100083,China;Excellence and Innovation Center,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China)

机构地区：[1]北京林业大学林木有害生物防治北京市重点实验室,北京100083 [2]江苏农业科学院分子卓越中心,南京210014

出　　处：《植物病理学报》2023年第2期173-182,共10页Acta Phytopathologica Sinica

基　　金：国家自然科学基金(31971657)。

摘　　要：大丽轮枝菌是世界范围的植物病原真菌,能够侵染660多种植物,可造成严重的经济损失。大丽轮枝菌基因组分析发现不同菌株含有菌株特异基因,但是大部分基因的生物学功能还不清楚。本研究通过比较大丽轮枝菌VdLs17和JR2的基因组鉴定出1个JR2菌株特异性基因Chr6g02370,该基因编码乙醇脱氢酶,且在侵染过程中被诱导表达。敲除突变体的乙醇脱氢酶酶活性显著降低,但对乙醇等碳源的利用能力并未受到影响。敲除突变体对烟草和番茄的致病力显著降低。表型分析表明敲除该基因降低了黑色素合成能力,但不影响菌丝生长和产孢。研究结果丰富了人们对大丽轮枝菌菌株特异基因生物学功能的认识,为深入开展其功能基因组学研究提供了遗传材料。Verticillium dahliae is a worldwide phytopathogenic fungus which can infect more than 660 plant species,causing serious economic losses annually.Genomic analysis of V.dahlia revealed that different strains contained many strain-specific genes,however,the biological functions of most genes were not clear.In our study,by comparing the genomes of V.dahliae VdLs17 and JR2,a JR2 strain-specific gene Chr6g02370 was identified,which encodes alcohol dehydrogenase and was induced during infection.The enzymatic activity of ethanol dehydrogenase in the knockout mutant decreased significantly,but the utilization of carbon sources such as ethanol was not affected.In addition,the virulence of knockout mutants to tobacco and tomato was significantly reduced.The phenotypic analysis also showed that knockout of this gene reduced melanin synthesis ability,but did not affect mycelial growth and sporulation.In conclusion,our results expand the understanding of the biological functions of strain-specific genes in V.dahlia and provide genetic material for further functional genomics.

关 键 词：大丽轮枝菌 菌株特异基因 乙醇脱氢酶 致病性 

分 类 号：S432.1[农业科学—植物病理学]