水稻MYB基因家族特征及其在RGSV和RRSV侵染后的响应  

Characteristics of the MYB transcription factors family and their responses to RGSV and RRSV infection in rice

作　　者：徐荣荣 常青 魏莹 李霞[1] 吴建国[1] 赵珊珊 XU Rongrong;CHANG Qing;WEI Ying;LI Xia;WU Jianguo;ZHAO Shanshan(Vector-borne Virus Research Center,Key Laboratory of Plant Virology of Fujian Province/State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops,Fujian Agriculture and Forestry University,Fuzhou 350002,China)

机构地区：[1]福建农林大学虫媒介病毒研究中心,福建省植物病毒学重点实验室,闽台作物有害生物生态防控国家重点实验室,福州350002

出　　处：《植物病理学报》2023年第2期258-266,共9页Acta Phytopathologica Sinica

基　　金：国家自然科学基金(32170167、32025031、U1905203)。

摘　　要：MYB基因家族作为植物体内最大的转录因子家族之一,在调节植物生长发育以及抗逆境胁迫方面发挥着至关重要的作用。然而,OsMYBs在水稻抗病毒通路中的作用还尚不清楚。为了探究OsMYBs家族在水稻抗病毒通路中所扮演的角色,对其结构、特征,以及对已报道的单链RNA病毒水稻草状矮化病毒(rice grassy stunt virus,RGSV)和双链RNA病毒水稻锯齿叶矮缩病毒(rice ragged stunt virus,RRSV)侵染后的表达谱进行分析,发现51个响应RGSV侵染,50个响应RRSV侵染。进一步分析发现,26个OsMYBs可同时响应两种不同类型病毒。从中筛选出10个表达量相对较高且差异倍数较大的基因进行实时荧光定量PCR(Real-time Quantitative PCR,qRT-PCR)验证,结果表明,OsMYB30、LOC_Os02g42850、LOC_Os02g47744、LOC_Os05g37050、LOC_Os06g43090和LOC_Os08g33150在响应两种病毒侵染后表达量下调;而LOC_Os05g38460在响应两种病毒侵染后表达量上调。LOC_Os05g51160和LOC_Os09g26170在RGSV侵染后表达量上调,在RRSV侵染后表达量下调;而LOC_Os08g05520在RGSV侵染后表达量下调,在RRSV侵染后表达量上调。这10个基因中有7个在两种病毒侵染后表达量变化趋势相同,表明这些基因可能对病毒响应具有广谱性。这为深入解析OsMYBs在水稻与病毒互作中的功能提供了有用的信息。As one of the largest transcription factor families in plants,MYB transcription factors family play important roles in regulating plant growth and development and resisting to stress.However,the role of OsMYBs in rice antiviral pathway is still unclear.In order to explore the roles of OsMYBs family in rice antiviral pathway,we first analyzed their structure and characteristics,as well as the expression profiles of single-stranded RNA virus rice grassy stunt virus(RGSV)and double-stranded RNA virus rice ragged stunt virus(RRSV)-infected rice published by Mohammed et al in 2015,and found that 51 of the OsMYBs family genes responded to RGSV and 50 of them responded to RRSV.Further analysis revealed that 26 OsMYBs responded to both RGSV and RRSV,and 10 of them with relatively high expression and multiple differences were selected for real-time fluorescence quantitative PCR(qRT-PCR)verification.Our results showed that the expression of OsMYB30,LOC_Os02g42850,LOC_Os02g47744,LOC_Os05g37050,LOC_Os06g43090 and LOC_Os08g33150 was down-regulated and the expression of LOC_Os05g38460 was up-regulated upon RGSV and RRSV infection,respectively.Moreover,the expression of LOC_Os05g51160 and LOC_Os09g26170 was up-regulated after RGSV infection while down-regulated after RRSV infection;Additionally,the expression of LOC_Os08g05520 was down-regulated after RGSV infection while up-regulated after RRSV infection.Seven out of these ten OsMYBs were identical in expression variation trend after infection of these two viruses,suggesting that these OsMYBs may have a broad-spectrum response to rice virus infection,which lays a foundation for future research on the function of OsMYBs during rice-virus interactions.

关 键 词：水稻MYB基因 水稻草状矮化病毒 水稻锯齿叶矮缩病毒 

分 类 号：S432.41[农业科学—植物病理学]