人参交链孢菌对氯氟醚菌唑的抗性风险评估  

Resistance risk assessment of mefentrifluconazole against Alternaria alternata from ginseng in China

作　　者：张嘉怡 冯志伟 侯万鹏 卢宝慧[1] 任广勇 孙国刚 高洁[1] ZHANG Jiayi;FENG Zhiwei;HOU Wanpeng;LU Baohui;REN Guangyong;SUN Guogang;GAO Jie(College of Plant Protection,Jilin Agricultural University,Changchun 130118,China;Jilin Shenwang Plant Protection Science and Technology Co.Ltd.,Baishan 134300,China;Ji'an Institute of Ginseng,Tonghua 134200,China)

机构地区：[1]吉林农业大学,长春130118 [2]吉林参王植保科技有限公司,白山134300 [3]集安市人参研究所,通化134200

出　　处：《植物病理学报》2023年第2期296-306,共11页Acta Phytopathologica Sinica

基　　金：吉林省科技发展计划(20191102039YY);中央本级重大增减支项目(2060302)。

摘　　要：为进一步了解人参交链孢菌(Alternaria alternata)对氯氟醚菌唑的抗药性风险,本研究通过药剂驯化法获得4株不同抗性水平的突变菌株,通过检测其遗传稳定性、生物学性状以及与其他杀菌剂间的交互抗性来进行A.alternata的抗性风险评估。结果表明高抗突变体适合度较高且能稳定遗传,一旦产生,有成为田间优势菌株的风险。氯氟醚菌唑抗药性突变体对氯氟醚菌唑的抗药性与对嘧菌酯、醚菌酯、苯醚甲环唑、吡唑醚菌酯和己唑醇5种药剂间不存在正交互抗性。A.alternata在药剂选择压力下可形成抗氯氟醚菌唑突变体,存在抗药性风险。本研究结果为吉林省人参黑斑病的防治及氯氟醚菌唑的合理使用提供科学依据。In order to understand the resistance risk of Alternaria alternata to mefentrifluconazole,four mutant strains with different resistance levels were obtained by chemical domestication in this study.The resistance risk of A.alternata was evaluated by detecting genetic stability,biological characteristics and cross resistance between mefentrifluconazole and other fungicides.The results showed that the high resistance mutant had high fitness and stable inheritance.Once it appears,it is at risk of becoming a dominant strain in the field.There was no positive cross resistance between the resistance of mefentrifluconazole resistant mutants to mefentrifluconazole and the resistance of other five fungicides,azoxystrobin,kersonxim-methyl,difenoconazole,pyraclostrobin,and hexaconazole.A.alternata can develop mefentrifluconazole-resistant mutants under the fungicide pressure,and there is a risk of fungicide resis-tance.The results of this study provide a scientific basis for the reasonable application of mefentrifluconazole for the control of ginseng Alternaria leaf blight.

关 键 词：人参 交链格孢菌 氯氟醚菌唑 生物学性状 抗药性风险评估 

分 类 号：S432.1[农业科学—植物病理学]