河南丹参棒孢霉叶斑病病原菌的鉴定  

Pathogen identification of the Corynespora leaf spot on Salvia miltiorrhiza Bge.in Henan

作　　者：王飞[1] 李雪梦 高素霞[1] 文艺[1] 秦艳红[1] 刘玉霞[1] 杨瑾 戚文平 鲁传涛[1] WANG Fei;LI Xuemeng;GAO Suxia;WEN Yi;QIN Yanhong;LIU Yuxia;YANG Jin;QI Wenping;LU Chuantao(Institute of Plant Protection,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China)

机构地区：[1]河南省农业科学院植物保护研究所,郑州450002

出　　处：《植物病理学报》2023年第2期317-320,共4页Acta Phytopathologica Sinica

基　　金：国家中药材产业技术体系(CARS-21);河南省农科院新兴学科(2020XK05、2021XK02、2022XK07);河南省农科院创新创意项目(2020CX10);河南省中药材产业技术体系(Z2019-13-01)。

摘　　要：丹参(Salvia miltiorrhiza Bge.),又叫活血根,为唇形科(Labiatae)鼠尾草属(Salvia Linn)多年生草本药用植物,干燥根入药,主要用于治疗心脑血管等疾病[1]。近年来,随着丹参种植规模的扩大,丹参根腐病[2-3]、枯萎病[4]和茎基腐[5]等病害的大规模发生,限制了丹参种植产业的发展。2019—2021年在河南省禹州、方城和三门峡丹参种植基地发现了一种与已报道的Alternaria zinnia Pape引起丹参叶斑病[6]症状不相同的叶部病害,该病害在河南产区发生普遍,为害重,田块发生率为100%,病株率为20%~70%。From 2019 to 2021,a new leaf spot disease was detected on Salvia miltiorrhiza Bge.in Henan province.The leaf spot disease could be found in all 12 investigated fields,and the rate of diseased plants was 20%to 70%.The disease spot was round or oval,gray white in the center,and appeared perforation at later stages which eventually led to leaf wilt by meeting together with multiple spots.The pathogen was isolated and purified by conventional tissue isolation,and was verified according to Koch′s rule.Based on morphological observation and rDNA-ITS,EF-1αandβ-tubulin gene sequence analysis,isolate DS1 was identified as Corynespora cassiicola,the causal agent of the new leaf spot disease.This is the first report of leaf spot disease on S.miltiorrhiza caused by C.cassiicola in China,which will provide theoretical basis and technical support for the effective control of the disease in the planting and production of S.miltiorrhiza in the future.

关 键 词：叶部病害 枯萎病 种植基地 棒孢霉叶斑病 鼠尾草属 唇形科 草本药用植物 

分 类 号：S432.1[农业科学—植物病理学]