水母雪莲乙醇提取物对Lewis肺癌荷瘤小鼠抗肿瘤免疫的影响  

Effect of ethanol extract from saussurea medusa maxim on anti-tumor immunity of Lewis lung cancer bearing mice

作　　者：张燕丽 岳秋娟 张林泉 张译 李鹏飞 张敏 ZHANG Yanli;YUE Qiujuan;ZHANG Linquan;ZHANG Yi;LI Pengfei;ZHANG Min(Key Laboratory for Molecular Genetic Mechanisms and Intervention Research on High Altitude Disease of Tibet Autonomous Region,Xianyang 712082,China)

机构地区：[1]西藏民族大学医学部高原相关疾病分子遗传机制与干预研究省级重点实验室,咸阳712082

出　　处：《中国免疫学杂志》2023年第5期988-992,998,共6页Chinese Journal of Immunology

基　　金：国家自然科学基金项目(81860719);西藏自治区自然科学基金项目(XZ2018ZRG-72);西藏民族大学校内科研资助项目(23MDY09)。

摘　　要：目的:初步探讨藏药水母雪莲(SMM)的体内外抗肿瘤免疫功能。方法:C57BL/6野生小鼠接种3LL细胞建立Lewis肺癌荷瘤小鼠模型,随机分为生理盐水组、SMM1低剂量组、SMM2中剂量组、SMM3高剂量组和环磷酰胺(CTX)组,体内连续治疗21 d。检测Lewis肺癌荷瘤小鼠实体瘤体积、质量和体质量;计算胸腺、脾脏指数;流式细胞仪检测肿瘤浸润组织淋巴细胞(TIL)的CD4^(+)T、CD8^(+)T百分比和CD4^(+)T/CD8^(+)T比例。体内MTT法检测80、100μg/ml SMM乙醇提取物对Lewis肺癌荷瘤小鼠脾CTL杀伤活性的影响;El ISA法检测CTL分泌IFN-γ的水平。结果:与生理盐水组比较,SMM乙醇提取物可显著抑制肿瘤生长并维持荷瘤小鼠体质量增长;胸腺和脾脏指数均显著升高(P<0.05);TIL中CD4^(+)T和CD4^(+)T/CD8^(+)T水平显著升高(P<0.05),SMM1和SMM3组CD8^(+)T显著降低(P<0.05),SMM2组差异无统计学意义。体外显著增强了荷瘤小鼠脾脏CTL的杀伤活性以及分泌IFN-γ的水平(P<0.01)。结论:SMM乙醇提取物可以通过增强免疫细胞功能发挥抗肿瘤免疫效应。Objective:To investigate the anti-tumor immune function of saussurea medusa maxim(SMM)in vitro and in vivo.Methods:A Lewis lung cancer bearing mice model by inoculating 3LL cells into C57BL/6 wild mice was established,and randomly assigned into normal saline group,SMM1 low-dose group,SMM2 medium-dose group,SMM3 high-dose group and cyclophosphamide(CTX)group.Mice were continuously treated for 21 d.The solid tumor volume,weight and body weight of Lewis lung cancer bearing mice,thymus and spleen index were detected.Percentage of CD4^(+)T,CD8^(+)T lymphocytes(TIL)and CD4^(+)T/CD8^(+)T ratio in tumor-infiltrating tissue were detected by flow cytometry.effect of 80、100μg/ml SMM ethanol extract on the cytotoxic activity of spleen CTL in Lewis tumor-bearing mice in vivo was detected by MTT method and ElISA was used to detecte IFN-γsecretion by CTL.Results:The treatment of SMM ethanol extract could significantly inhibit tumor growth and maintain the normal body weight of mice.Thymus index and spleen index were both increased significantly(P<0.05).SMM ethanol extract significantly increased the proportion of tumor infiltrating CD4^(+)T cells and the ratio of CD4^(+)T/CD8^(+)T in TIL(P<0.05).CD8^(+)T in SMM1 and SMM3 groups decreased significantly(P<0.05),but there was no significant difference in SMM2 group.In addition,the cytotoxic activity of spleen CTL and the secretion of IFN-γin tumor-bearing mice were significantly enhanced in vitro(P<0.01).Conclusion:The ethanol extract of SMM could achieve anti-tumor immune effect by enhancing the function of immune cells.

关 键 词：水母雪莲 T淋巴细胞亚群 细胞因子 抗肿瘤免疫 

分 类 号：R285.5[医药卫生—中药学]