Quality Evaluation of Streptocaulon griffithii Hook Based on Fingerprint and Quantitative Analysis of Multiple Components by the QAMS Method  

作　　者：Xinying MO Honghan QIN Yudan LU Baoxian LIANG Hongsheng TAN Haisheng ZENG 

机构地区：[1]The Affiliated Hospital of Youjiang Medical University for Nationalities,Baise 533000,China [2]Youjiang Medical University for Nationalities,Baise 533000,China [3]Institute of Physiology and Ecology of Medicinal Plants/College of Traditional Chinese Medicine,Guangzhou University of Chinese Medicine,Guangzhou 510006,China

出　　处：《Medicinal Plant》2023年第2期46-51,共6页药用植物：英文版

基　　金：Supported by Scientific Research Project of Guangxi Zhuang Autonomous Region Administration of Traditional Chinese Medicine(GZZC2019147);2020 Guangxi University Young and Middle-aged Teachers'Basic Scientific Research Ability Improvement Project(2020KY13034);the First Scientific Research Project of High-level Talents in the Affiliated Hospital of Youjiang Medical University for Nationalities in 2019(Y20196311);Scientific Research Project of Guangxi Zhuang Autonomous Region Administration of Traditional Chinese Medicine(20210241)。

摘　　要：[Objectives]Chromatographic fingerprint analysis technology and quantitative analysis of multi-components by singlemarker(QAMS)were used to identify the authenticity and multi-component quantitative analysis ofStreptocaulon griffithiiHook,which provided experimental reference for the quality evaluation and control ofS.griffithiiHook.[Methods]Method was carried out on Agilent ZORBAX SB-C18(150 mm×4.6 mm,5μm)column with mobile phase composed of methanol-0.2%phosphoric acid solution at a flow rate of 1.0 mL/min in gradient elution mode.The column temperature was maintained at 30℃,the injection volume was 5μL,and the detection wavelengths were set at 230 nm.The HPLC fingerprint ofS.griffithiiHook was established byFingerprint Similarity Evaluation Software of Traditional Chinese Med-i cine(2012 edition),and the quality of 11 batches ofS.griffithiiHook extracts was analyzed.The content of chlorogenic acid,caffeic acid and4-methoxysalicylaldehyde inS.griffithiiHook was determined by QAMS.[Results]Thirteen common peaks were identified in the extract ofS.g riffithiiHook,and three components were identified as chlorogenic acid,caffeic acid and 4-methoxysalicylaldehyde,there was no significant difference between QAMS method and external standard method with chlorogenic acid as reference substance.[Conclusions]The established HPLC method is specific,accurate,stable and reproducible,and it can be used as an effective method for the quality control ofS.griffithii Hook.

关 键 词：Streptocaulon griffithiiHook FINGERPRINT Content determination QAMS CHEMOMETRICS 

分 类 号：R284.1[医药卫生—中药学]