Establishment of Molecular Biological Method for Identification of Bacteria by 16S rDNA and gyrB Gene  

作　　者：Xiaxia HOU Yunxia WANG Cuizhi LI Zhiyong LU 

机构地区：[1]Inner Mongolia Yili Industrial Group Co.,Ltd.,Hohhot 010110,China

出　　处：《Asian Agricultural Research》2023年第4期21-25,共5页亚洲农业研究（英文）

基　　金：Supported by Special Project of"Grassland Talents"in Inner Mongolia.

摘　　要：[Objectives]The paper was to establish a molecular biological method for identification of bacterial strains.[Methods]The thalli of standard bacterial strains existing in the laboratory were collected and genomic DNA was extracted for amplification of 16S rDNA and gyrB gene.The 16S rDNA and gyrB gene sequences were obtained after sequencing.Sequences were aligned and analyzed via EzBioCloud and NCBI database,and phylogenetic trees were constructed to determine the species relationship of strains.Meantime,they were compared with known strains.[Results]This method could identify 5 standard strains accurately to the species level.The 16S rDNA and gyrB gene sequences were aligned and analyzed in EzBioCloud database and NCBI database.The strain with the max score was consistent with the known strain.And the query cover and ident were both above 99%.[Conclusions]The established molecular biological method for identification of bacterial strains by 16S rDNA and gyrB gene has good accuracy,which effectively solves the problem that the laboratory identification of bacteria relies on traditional methods and the accuracy can not be guaranteed,and further improves the identification ability of laboratory bacterial strains.

关 键 词：16S rDNA GYRB Bacterial identification Molecular biological method 

分 类 号：Q93-331[生物学—微生物学]