黄柄曲霉的次生代谢产物分析及抗肿瘤活性研究  

Analysis of Secondary Metabolites and Anti-Tumor Activity Aspergillus Flavus

作　　者：杨丽军 方申超 夏楠 颜廷旋 YANG Lijun;FANG Shenchao;XIA Nan;YAN Tingxuan(School of Food ang Biological Engineering,Wuhu Institute of Technology,Wuhu,Anhui 241000;Wuhu Agricultural Products and Food Testing Center,Wuhu,Anhui 241000;College of Biological and Environmental Engineering,Binzhou University,Binzhou,Shandong 256600)

机构地区：[1]芜湖职业技术学院食品与生物工程学院,安徽芜湖241000 [2]芜湖市农产品食品检测中心,安徽芜湖241000 [3]滨州学院生物与环境工程学院,山东滨州256600

出　　处：《绵阳师范学院学报》2023年第5期45-52,共8页Journal of Mianyang Teachers＇ College

基　　金：芜湖职业技术学院自然科学研究一般项目(wzyzr202232);安徽省高等学校省级自然科学研究重点项目(KJ2016A765);安徽省质量工程校企合作实践教育基地(2020sjjd139).

摘　　要：以富产细胞松弛素的黄柄曲霉为研究对象,对其追踪分离,从中获得单体细胞松弛素类化合物,并进行抗肿瘤抗菌活性测试,期待发现结构新颖、活性显著的细胞松弛素类化合物,为抗肿瘤抗菌药物研发提供模板分子.研究采用PDA培养基分离黄柄曲霉菌,用真菌2号培养基发酵,乙酸乙酯萃取发酵物,经硅胶色谱、ODS、凝胶柱色谱分离纯化,再通过高效液相色谱进行单体化合物的制备.利用核磁共振、质谱解析化合物结构后,选择肿瘤细胞A549、H1299为实验对象,采用CCK8法测定单体化合物的抗肿瘤活性.结果表明化合物1、化合物2、化合物3对肿瘤细胞A549、H1299具有一定的细胞毒性,其中化合物1(Cytochalasin E)具有较强的细胞毒性,可以较好的诱导肿瘤细胞凋亡.This study focused on the filamentous fungus Fusarium oxysporum,which is rich in cell-relaxing agents.The objective was to isolate and identify monomeric cell-relaxing compounds and test their anti-tumor and antibacterial activities.The goal was to discover novel and potent cell-relaxing compounds that could serve as template molecules for the development of anti-tumor and antibacterial drugs.The fungus was isolated using PDA medium,fermented using Fungi No.2 medium,and the fermentation product was extracted using ethyl acetate.The extract was then purified using silica gel,ODS,and gel column chromatography,and monomeric compounds were prepared using high-performance liquid chromatography.After analyzing the structure of the compounds using nuclear magnetic resonance and mass spectrometry,tumor cells A549 and H1299 were selected as experimental subjects,and the anti-tumor activity of the monomeric compounds was determined using the CCK8 method.The results showed that compound 1,compound 2,and compound 3 exhibited certain cytotoxicity against tumor cells A549 and H1299,and compound 1,cytochalasin E,demonstrated strong cytotoxicity and was effective in inducing tumor cell apoptosis.

关 键 词：黄柄曲霉 细胞松弛素 抗肿瘤活性 

分 类 号：R284[医药卫生—中药学]