一株多粘类芽孢杆菌的鉴定及其对花椒根腐病菌Fusarium solani的抑菌作用  

Identification of a PaenibacillusPolymyxastrain and its Antibacterial Effect on Root Rotin Zanthoxylum Bungeanum ofFusarium Solani

作　　者：卓平清 王瀚[1,2] 王昱 赵淑玲[1,2] 王林林 王弋博[3] 田凤鸣 ZHUO Pingqing;WANG Han;WANG Yu;ZHAO Shuling;WANG Linlin;WANG Yibo;TIAN Fengming(School of Life Science,Longnan Teachers'College,Longnan,Gansu 742500;Center for Research and Development of Longnan Characteristic Agro-Bioresource,Longnan,Gansu 742500;College of Bioengineering and Biotechnology,Tianshui Normal University,Tianshui,Gansu 741001)

机构地区：[1]陇南师范高等专科学校农林技术学院,甘肃陇南742500 [2]陇南特色农业生物资源研究开发中心,甘肃陇南742500 [3]天水师范学院生物工程与技术学院,甘肃天水741000

出　　处：《绵阳师范学院学报》2023年第5期68-74,共7页Journal of Mianyang Teachers＇ College

基　　金：国家自然科学基金项目(31960268);甘肃省高等学校科研创新基金项目(2020A-272);2020年陇南市科技指导性计划项目(2020-ZD-05).

摘　　要：腐皮镰刀菌(Fusarium solani)是引起花椒根腐病的主要病原菌之一,为开发高效防治花椒根腐病的绿色生防菌,实验从健康花椒根部土壤中分离筛选出一株腐皮镰刀菌较高拮抗作用的细菌KS-16,并对其进行形态学、生理生化性质和分子鉴定,研究了该菌对三株供试菌株大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌及腐皮镰刀菌的抑菌作用.结果表明,菌株KS-16被鉴定为多粘类芽孢杆菌(Paenibacillus polymyxa).活性测定发现,该菌对腐皮镰刀菌的菌丝生长抑制率达到53.78%,对三株供试菌株也有较高的抑菌活性.以PDA培养基作为发酵培养基优于LB培养基,作用10 d其抑制效果仍保持在43.33%.Fusarium solaniis one of the main pathogens causing root rot in Zanthoxylum bungeanum.In order to develop a green biocontrol strain for effective prevention and control of root rot of Zanthoxylum bungeanum,a strain KS-16 with highly antagonistic to F.solani was isolated from healthy Zanthoxylum bungeanum root soil,morphological and physiological and biochemical characteristics and molecular identification of the antibacterial effect of this bacterium against Escherichia coli,Staphylococcus aureus,Bacillus subtilis and Fusariumsolani were researched.The strain KS-16 was identified as Paenibacilluspolymyxa.The activity test showed that the strain had an inhibition rate of 53.78%against the mycelial growth of F.solani and had high antibacterial activity effect on the three strains,and the inhibition rate remained at 43.33%after 10 days while the PDA medium as fermentation medium was better than LB medium.

关 键 词：花椒 根腐病 腐皮镰刀菌 多粘类芽孢杆菌 抑菌活性 

分 类 号：S476[农业科学—农业昆虫与害虫防治]