Transcriptomic analysis of Andrias davidianus meat and experimental validation for exploring its bioactive components as functional foods  

作　　者：Changge Guan Zhenglin Tan Shucheng Li Yi Wang Naoyuki Yamamoto Chong Zhang Songjun Wang Junjie Chen Xinhui Xing 

机构地区：[1]Key Laboratory for Industrial Biocatalysis,Ministry of Education of China,Department of Chemical Engineering,Tsinghua University,Beijing 100084,China [2]School of Life Science and Technology,Tokyo Institute of Technology,Yokohama 226-0026,Japan [3]Sensing System Research Center,National Institute of Advanced Industrial Science and Technology,Tsukuba 305-8565,Japan [4]Center for Synthetic and Systems Biology,Tsinghua University,Beijing 100084,China [5]Henan Giant Salamander Protection and Development Association,Luoyang 471000,China [6]Intelligent Computing Research Center,Harbin Institute of Technology,Shenzhen 518071,China [7]Institute of Biopharmaceutical and Health Engineering,Tsinghua Shenzhen International Graduate School,Shenzhen 518055,China [8]Institute of Biomedical Health Technology and Engineering,Shenzhen Bay Laboratory,Shenzhen 518067,China

出　　处：《Food Science and Human Wellness》2024年第1期166-172,共7页食品科学与人类健康（英文）

基　　金：funded by Shenzhen Science and Technology Innovation Commission(KCXFZ20201221173207022)。

摘　　要：Andrias davidianus(Chinese giant salamander,CGS)is the largest and oldest extant amphibian species in the world and is a source of prospective functional food in China.However,the progress of functional peptides mining was slow due to lack of reference genome and protein sequence data.In this study,we illustrated full-length transcriptome sequencing to interpret the proteome of CGS meat and obtain 10703 coding DNA sequences.By functional annotation and amino acid composition analysis,we have discovered various genes related to signal transduction,and 16 genes related to longevity.We have also found vast variety of functional peptides through protein coding sequence(CDS)analysis by comparing the data obtained with the functional peptide database.Val-Pro-Ile predicted by the CDS analysis was released from the CGS meat through enzymatic hydrolysis,suggesting that our approach is reliable.This study suggested that transcriptomic analysis can be used as a reference to guide polypeptide mining in CGS meat,thereby providing a powerful mining strategy for the bioresources with unknown genomic and proteomic sequences.

关 键 词：Chinese giant salamander Transcriptomic analysis Bioactive components Functional peptides mining 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]