Proximity Chemistry in Living Systems  

作　　者：Yanjun Liu Yun Ge Ruxin Zeng William Shu Ching Ngai Xinyuan Fan Peng R.Chen 

机构地区：[1]Synthetic and Functional Biomolecules Center,Beijing National Laboratory for Molecular Sciences,Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education,College of Chemistry and Molecular Engineering,Peking University,Beijing 100871 [2]National Key Laboratory of Crop Genetic Improvement,Hubei Hongshan Laboratory,Huazhong Agricultural University,Wuhan 430070 [3]Shenzhen Bay Laboratory,Institute of Chemical Biology,Shenzhen 518107

出　　处：《CCS Chemistry》2023年第4期802-813,共12页中国化学会会刊（英文）

基　　金：funding from the National Natural Science Foundation of China(grant nos.21937001,22222701,22137001,91957101,and 22077004);the Ministry of Science and Technology of China(grant nos.2022YFA1304700,2019YFA0904201,and 2021YFA1302603);Beijing Natural Science Foundation(grant no.Z200010).

摘　　要：Enzyme-and catalyst-generated reactive species have been leveraged in the past decade to covalently label biomolecules within a short range of a defined site or space inside cells or at the cell–cell interface.Due to their high spatial resolution,such proximity labeling strategies have been coupled with various bioanalytical techniques for dissecting dynamic and complex biological processes.Here,we review the development of enzyme-and catalyst-triggered proximity chemistry and their applications to identifying protein interaction networks as well as cell–cell communications in living systems.

关 键 词：proximity labeling bioorthogonal chemistry spatiotemporal proteomics cell-cell interactions living systems 

分 类 号：O6[理学—化学]