白花除虫菊高效再生体系的建立  

High-performance Regeneration of Pyrethrum

作　　者：张艺萍[1] 张丽芳[1] 许凤[1] 王丽花[1] 苏艳[1] 杨秀梅[1] 蒋亚莲[1] ZHANG Yiping;ZHANG Lifang;XU Feng;WANG Lihua;SU Yan;YANG Xiumei;JIANG Yalian(Flower Research Institute,Yunnan Academy of Agricultural Sciences/National Engineering Research Center for Ornamental Horticulture/Yunnan Key Laboratory of Flower Breeding/Yunnan Flower Engineering Research Center for Flower/Kunming Key Laboratory of Genetic Improvement of Flowers,Kunming,Yunnan 650205,China)

机构地区：[1]云南省农业科学院花卉研究所/国家观赏园艺工程研究中心/云南省花卉育种重点实验室/云南省花卉工程技术研究中心/昆明市花卉遗传改良重点实验室,云南昆明650205

出　　处：《福建农业学报》2023年第3期346-351,共6页Fujian Journal of Agricultural Sciences

基　　金：云南省重点新产品开发专项(2016BB009)。

摘　　要：【目的】筛选出适宜白花除虫菊外植体的消毒方法、外植体诱导、继代培养及生根培养阶段适宜的培养基配方,建立白花除虫菊高效再生体系。【方法】以白花除虫菊尚未张开的花蕾作为再生体系的外植体材料,花蕾经过灭菌消毒以后,采用MS固体培养基,比较不同种类植物生长调节剂浓度配比对白花除虫菊花蕾诱导出芽、增殖及生根等关键环节的影响。【结果】白花除虫菊花蕾外植体最适宜的消毒条件为75%酒精处理30 s后用0.10%氯化汞溶液消毒10 min,之后用15%次氯酸溶液处理15 min;白花除虫菊花蕾诱导芽的最适培养基为MS+2.0 mg·L^(-1)6-BA+0.5 mg·L^(-1)TDZ+0.2 mg·L^(-1)IBA;白花除虫菊芽增殖的最适培养基为MS+1.0 mg·L^(-1)6-BA+0.1 mg·L^(-1)TDZ+0.1 mg·L^(-1)IBA;白花除虫菊生根的最适培养基为MS+0.1 mg·L^(-1)IAA+0.1 mg·L^(-1)IBA;炼苗移栽的最适基质为泥炭∶珍珠岩=6∶1,成活率达90%以上,移栽效果良好。【结论】基本建立了白花除虫菊高效再生体系,可为繁育白花除虫菊种苗提供技术支撑。【Objective】Appropriate methods for efficient tissue disinfection,explant induction,and culture medium formulation were established for a high-performance program to propagate pyrethrum.【Method】Unopened flower buds of white flower pyrethrum were sterilized and used as explants on an MS solid medium for the experiment.Effects of various plant growth regulators on bud induction,proliferation,and rooting of the seedlings were monitored.【Result】The optimum conditions for disinfecting the explants were found to be a treatment of 75%alcohol for 30 s followed by one of 0.10%mercuric chloride solution for 10 min and another of 15%hypochlorite for 15 min.For bud induction,the choice medium was formulated with MS+2.0 mg·L^(-1)6-BA+0.5 mg·L^(-1) TDZ+0.2 mg·L^(-1) IBA;for bud proliferation,MS+1.0 mg·L^(-1)6-BA+0.1 mg·L^(-1)TDZ+0.1 mg·L^(-1) IBA;for rooting,MS+0.1 mg·L^(-1) IAA+0.1 mg·L^(-1) IBA;and for transplanting seedlings,peat:perlite at 6:1.A survival rate greater than 90%as well as adequate transplanting was achieved.【Conclusion】The newly developed in vitro regeneration system materially lessened the pressure of the recently encountered white flower pyrethrum germplasm degradation.In addition,a supply of high-quality seedlings could be assured with the proposed propagation program.

关 键 词：白花除虫菊 不定芽 再生体系 

分 类 号：S682.31[农业科学—观赏园艺]