基于HPLC指纹特征关联O2PLS-DA模型分析低温干燥当归药材、饮片、标准汤剂物质基础差异  

Analysis on Material Basis Differences of Low-Temperature Dried Danggui(Angelicae Sinensis Radix),Decoction Pieces and Standard Decoction Based on HPLC Fingerprint Characteristics and O2PLS-DA Model

作　　者：李波[1] 吴嘉慧 黄晶[1] 卢章宁 黄柳楠 梁燕 陈杰 高雅[1] 张可锋[1,3] LI Bo;WU Jiahui;HUANG Jing;LU Zhangning;HUANG Liunan;LIANG Yan;CHEN Jie;GAO Ya;ZHANG Kefeng(Guilin Medical University,Guilin 541004,Guangxi,Chnia;Lintan Tianshili Zhongtian Pharmaceutical Co.,Ltd.,Gannan 747500,Gansu,Chnia;Key Laboratory of High Incidence Prevention and Treatment Pharmacology,Guilin Medical University,Guilin 541004,Guangxi,Chnia)

机构地区：[1]桂林医学院,广西桂林541004 [2]临潭天士力中天药业有限责任公司,甘肃甘南州747500 [3]桂林医学院高发病防治药理学重点实验室,广西桂林541004

出　　处：《中华中医药学刊》2023年第4期120-125,I0030,I0031,共8页Chinese Archives of Traditional Chinese Medicine

基　　金：国家中药标准化项目(ZYBZH-Y-GS-11);广西高校中青年教师科研基础能力提升项目(2021KY0500)。

摘　　要：目的基于HPLC指纹特征整合O2PLS-DA模型,阐明低温(阴干、30→60℃)干燥、加工(切片)和煎煮对当归临床药用药物质基础影响的关系。方法采用HPLC指纹特征整合O2PLS-DA模型、SPSS统计等方法,分析低温干燥(阴干、30→60℃)、切片、煎煮对当归药材及其制备的饮片、标准汤剂HPLC指纹特征差异的影响和主要差异成分的相关性。结果当归低温(阴干、30→60℃)药材、饮片、标准汤剂HPLC指纹特征相似度高,分别在0.979~0.998、0.890~1、0.864~0.994。鲜切当归及其制备标准汤剂与低温干燥组相似度低,分别在0.890~0.910与0.530~0.792。当归饮片及标准汤剂HPLC指纹特征O2PLS-DA模型聚类为3组:阴干和30~50℃组、60℃组、鲜切组。当归药材、饮片、标准汤剂HPLC指纹特征O2PLS-DA模型VIP值:8号峰>3号峰>4号峰>6号峰>7号峰>5号峰>1号峰>2号峰,指认出藁本内酯(8号峰)、绿原酸(3号峰)、阿魏酸(4号峰)(VIP>0.88)为关键差异成分。Pearson分析显示阿魏酸和绿原酸含量呈正相关(P<0.01),与藁本内酯含量呈负相关(P<0.01)。结论低温干燥当归药材指标成分符合《中华人民共和国药典》规定,干燥、加工和煎煮对当归物质基础影响趋势不同。Objective Based on HPLC fingerprint characteristics and O2PLS-DA model,to clarify the effects of low temperature(shade drying,30℃→60℃)drying and processing(slice,decocting)on the material basis of Danggui(Angelicae Sinensis Radix),decoction pieces and standard decoction.Method HPLC fingerprint characteristics were integrated with O2PLS-DA model,SPSS statistics and other methods to analyze the influence of low temperature drying(shade drying,30℃→60℃),slicing,decocting on the difference of HPLC fingerprint characteristics of Danggui(Angelicae Sinensis Radix)and its prepared decoction pieces and standard decoction and the correlation of the main difference components.Results HPLC fingerprint characteristics of low temperature(shade drying,30℃→60℃)of Danggui(Angelicae Sinensis Radix),decoction pieces and standard decoction had high similarity,which ranged from 0.979~0.998,0.890~1 and 0.864~0.994,respectively.The similarity between fresh cut Danggui(Angelicae Sinensis Radix)and its preparation standard decoction and low temperature drying group was low,between 0.890~0.910 and 0.530~0.792,respectively.HPLC fingerprint characteristics of Danggui(Angelicae Sinensis Radix)decoction pieces and standard decoction O2PLS-DA model clustering was divided into three groups:shade drying and 30~50℃group,60℃group,fresh cut group.HPLC fingerprint characteristics of Danggui(Angelicae Sinensis Radix),decoction pieces and standard decoction O2PLS-DA model VIP value:peak 8>peak3>peak 4>peak 6>peak 7>peak 5>peak 1>peak 2 indicated that ligustilide(peak 8),chlorogenic acid(peak 3)and ferulic acid(peak 4)(VI P>0.88)were identified as the key differential components.Pearson analysis showed that the contents of ferulic acid and chlorogenic acid were positively correlated(P<0.01),but negatively correlated with ligustilide content(P<0.01).Conclusion The index components of Danggui(Angelicae Sinensis Radix)dried at low temperature meet the provisions of Chinese Pharmacopoeia,but the influence trend of drying,processing and

关 键 词：当归药材 低温干燥 饮片 标准汤剂 HPLC指纹 O2PLS-DA 物质基础 

分 类 号：R284.1[医药卫生—中药学]