不同产地棒毛络新妇蜘蛛的脂溶性成分GC-MS分析  

GC-MS Analysis of Liposoluble Constituent of Bangmaoluoxinfu(Trichonephila clavata)Spider from Different Regions

作　　者：顾小亮 杨月娥 杨志斌 罗建蓉 杨自忠 GU Xiaoliang;YANG Yuee;YANG Zhibin;LUO Jianrong;YANG Zizhong(Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D,Dali University,Dali 671000,Yunnan,China;National-Local Joint Engineering Research Center of Entomoceutics,Dali University,Dali 671000,Yunnan,China;Innovative Team of Dali University for Medicinal Insects&Arachnids Resources Digital Development,Dali 671000,Yunnan,China;College of Pharmacy,Dali University,Dali 671000,Yunnan,China)

机构地区：[1]大理大学云南省昆虫生物医药研发重点实验室,云南大理671000 [2]大理大学药用特种昆虫开发国家地方联合工程研究中心,云南大理671000 [3]大理大学药用昆虫及蛛形类生物资源数字化开发创新团队,云南大理671000 [4]大理大学药学院,云南大理671000

出　　处：《中华中医药学刊》2023年第4期239-243,I0051,I0052,共7页Chinese Archives of Traditional Chinese Medicine

基　　金：云南省重大科技专项计划项目(202002AA100007)。

摘　　要：目的研究不同产地棒毛络新妇Trichonephila clavata蜘蛛的脂溶性成分,比较不同产地棒毛络新妇脂溶性成分组成和含量的差异。方法样品以石油醚进行索氏提取得到脂溶性成分,采用气相色谱质谱联用仪对甲酯化的样品进行检测,最后通过标准谱库(NIST)进行检索和鉴定,用峰面积归一化法计算相对百分含量,并采用聚类分析、主成分分析和相似度评价对整体数据进行分析。结果棒毛络新妇脂溶性成分提取率为12.25%~16.10%,从中共鉴定出脂溶性成分26种,共有成分为11种,以油酸、亚油酸和棕榈酸含量最高,平均含量分别为35.84%、20.43%和11.87%;聚类分析和主成分分析结果可按产地分类,相似度评价结果在0.383~0.983。结论棒毛络新妇脂溶性成分的种类及相对百分含量受产地和生境的影响。研究为棒毛络新妇的物质基础研究提供实验数据,同时也为棒毛络新妇作为食品开发利用提供依据。Objective To study the liposoluble components of Bangmaoluoxinfu(Trichonephila clavata)from different producing areas and compare the composition and content of lipid-soluble components of Bangmaoluoxinfu(Trichonephila clavata)from different areas.Methods The samples were soxhlet extracted with petroleum ether to obtain liposoluble components,and the samples were detected by GC-MS.Finally,the index was retrieved and identified by standard spectrum library(NIST).The relative percentage was calculated by peak area normalization method,and the cluster analysis,principal component analysis and similarity evalution were used to analyze the whole data.Results The extraction rate of liposoluble components was 12.25%-16.10%.A total of 26 liposoluble components were identified from extraction of Bangmaoluoxinfu(Trichonephila clavata),of which were 11 co-owned components,and the contents of oleic acid,linoleic acid and palmitic acid were the highest,and the average contents were 35.84%,20.43%and 11.87%,respectively.The results of cluster analysis and principal component analysis could be classified by place of origin,and the similarity evaluation results ranged from 0.383 to 0.983.Conclusion The species and relative percentage of lipid-soluble components of Bangmaoluoxinfu(Trichonephila clavata)were affected by producing areas and habitat.This study provides basic data for the study of material basis and scientific data for the development and utilization of Bangmaoluoxinfu(Trichonephila clavata)as food.

关 键 词：棒毛络新妇 脂溶性成分 气相色谱-质谱联用分析 脂肪酸 

分 类 号：R284.1[医药卫生—中药学]