IL-8及IFN-γ基因多态性与扁桃体摘除术后感染的关联  

Association of gene polymorphisms of IL-8 and IFN-γwith postoperative infection in tonsillectomy patients

作　　者：曾萍 吴璇[1] 张艳 陶亚玲[1] 吴敏[1] 陈珍珍[1] 孟君[1] 张程程 ZENG Ping;WU Xuan;ZHANG Yan;TAO Ya-ling;WU Min;CHEN Zhen-zhen;MENG Jun;ZHANG Cheng-cheng(Zaoyang First People's Hospital,Zaoyang,Hubei 441200,China)

机构地区：[1]枣阳市第一人民医院耳鼻咽喉科,湖北枣阳441200

出　　处：《中华医院感染学杂志》2023年第9期1366-1370,共5页Chinese Journal of Nosocomiology

基　　金：湖北省自然科学基金项目(2019CFB326)。

摘　　要：目的探讨白细胞介素-8(IL-8)、干扰素-γ(IFN-γ)基因多态性与扁桃体摘除术后感染易感性的关系。方法选取2019年4月-2021年4月枣阳市第一人民医院耳鼻咽喉科收治的198例行扁桃体摘除术患者为研究对象,根据是否发生术后感染分为感染组59例和非感染组139例,采用聚合酶链反应(PCR)及测序技术检测IL-8基因-251T/A、+781C/T位点及IFN-γ基因+874A/T位点基因分布,比较两组患者炎症因子[肿瘤坏死因子-α(TNF-α)、C-反应蛋白(CRP)]、免疫功能指标[免疫球蛋白G(IgG)、IgM]以及血清IL-8、IFN-γ水平。结果感染组患者吸烟、饮酒史占比高于非感染组(P<0.05);感染组患者血清CRP、TNF-α、IL-8、IFN-γ水平均高于非感染组,IgA、IgM水平低于非感染组(P<0.05);感染组患者IL-8基因-251T/A位点AA基因型及A等位基因频率均高于非感染组(P<0.05);感染组IL-8基因-251T/A位点AA基因型患者血清IL-8水平高于AT及TT基因型患者(P<0.05)。结论扁桃体摘除术后感染患者免疫功能降低、炎症反应明显,IL-8基因-251T/A位点AA基因型及A等位基因能增加扁桃体摘除术后感染发生率。OBJECTIVE To explore the association of gene polymorphisms of interleukin-8(IL-8)and interferon-γ(IFN-γ)with susceptibility to postoperative infection in the tonsillectomy patients.METHODS A total of 198 patients who underwent tonsillectomy in otolaryngology department of Zaoyang First People′s Hospital from Apr 2019 to Apr 2021 were recruited as the research subjects and divided into the infection group with 59 cases and the no infection group with 139 cases according to the status of postoperative infection.The genotypes at IL-8 gene-251T/A,+781C/T loci and IFN-γgene+874A/T loci were detected by polymerase chain reaction(PCR)and sequencing technique.The levels of inflammatory factors[tumor necrosis factor-α(TNF-α),C-reactive protein(CRP)],immune function indexes[immunoglobulin G(IgG)、IgM],serum IL-8 and IFN-γwere observed and compared between the two groups of patients.RESULTS The proportion of the patients with history of smoking and drinking were higher in the infection group than in the no infection group(P<0.05).The levels of serum CRP,TNF-α,IL-8 and IFN-γof the infection group were higher than those of the no infection group,while the levels of IgA and IgM of the infection group were lower than those of the no infection group(P<0.05).The frequencies of AA genotype and A allele at IL-8 gene-251T/A locus were higher in the infection group than in the no infection group(P<0.05).In the infection group,the levels of serum IL-8 of the patients with AA genotype at IL-8 gene-251T/A locus were higher than those of the patients with AT genotype and TT genotype(P<0.05).CONCLUSION The tonsillectomy patients with postoperative infection show the decline of immune function and remarkable inflammatory response.The AA genotype and A allele at IL-8 gene-251T/A locus may increase the incidence of postoperative infection in the tonsillectomy patients.

关 键 词：白细胞介素-8 干扰素-Γ 基因多态性 扁桃体摘除术 术后感染 

分 类 号：R766.18[医药卫生—耳鼻咽喉科]