胸腔积液患者胸水和血清中IL-33和sST2表达水平与炎症反应的相关性分析  

Correlation analysis of IL-33 and sST2 expression levels in pleural effusion and serum with inflammatory response in patients with pleural effusion.

作　　者：白峰[1] 张小雷 彭海军[1] 王明正[1] 刘佳坤[1] 王维[1] 韩青松[1] 栾艳超[1] BAI Feng;ZHANG Xiao-lei;PENG Hai-jun(Department of Chest Surgery,Hebei Province Chest Hospital/Hebei Provincial Key Laboratory of Lung Disease,Shijiazhuang Hebei 050000,China;Department of General Internal Medicine,The Eighth People's Hospital of Hebei Province,Shijiazhuang Hebei 050051,China)

机构地区：[1]河北省胸科医院/河北省肺病重点实验室胸外科,河北石家庄050000 [2]河北省第八人民医院普内科,河北石家庄050051

出　　处：《临床和实验医学杂志》2023年第8期825-828,共4页Journal of Clinical and Experimental Medicine

基　　金：河北省医学科学研究重点课题计划(编号:20180651)。

摘　　要：目的研究胸腔积液患者胸水和血清中白细胞介素(IL)-33和可溶性生长刺激表达基因2蛋白(sST2)表达水平与炎症反应的相关性。方法回顾性纳入2019年3月至2022年3月河北省胸科医院收治的108例胸腔积液患者作为研究对象,其中结核性胸腔积液(TPE)患者作为TPE组(n=45)、恶性胸腔积液(MPE)患者作为MPE组(n=60),另于同期选择50名在本院体检的健康成年人作为对照组(n=50)。检测胸水IL-33、sST2水平及血清IL-33、sST2、IL-6、C反应蛋白(CRP)、肿瘤坏死因子-α(TNF-α)水平。以Pearson相关系数分析TPE和MPE患者胸水和血清IL-33和sST2与血清炎症因子(IL-6、CRP、TNF-α)的相关性;以受试者工作特征(ROC)曲线分析胸水和血清IL-33和sST2诊断TPE的价值。结果TPE组患者胸水IL-33和sST2水平分别为(83.52±21.09)、(1108.37±236.15)ng/L,均高于MPE组[(53.16±11.24)、(726.49±151.28)ng/L],差异均有统计学意义(P<0.05);TPE组和MPE组血清IL-33和sST2水平均高于对照组,并且TPE组分别为(74.13±18.49)、(1454.07±477.19)ng/L,均高于MPE组[(58.46±16.52)、(1228.65±374.64)ng/L],差异均有统计学意义(P<0.05)。3组血清IL-6、CRP、TNF-α水平比较,TPE组和MPE组高于对照组,并且TPE组高于MPE组,差异均有统计学意义(P<0.05)。TPE患者胸水和血清IL-33和sST2与血清IL-6、CRP、TNF-α水平呈正相关(P<0.05);MPE患者胸水和血清IL-33和sST2与血清IL-6、CRP、TNF-α水平呈正相关(P<0.05)。经ROC分析,胸水IL-33≥69.142 ng/L、胸水sST2≤943.715 ng/L、血清IL-33≥65.172 ng/L、血清sST2≥1308.664 ng/L是诊断TPE的最佳截断值(P<0.05)。结论胸水和血清IL-33和sST2与TPE和MPE的发生、发展密切相关,并与IL-6、CRP、TNF-α等炎症因子水平呈正相关,临床可通过检测胸水和血清IL-33和sST2鉴别TPE和MPE。Objective To investigate the correlation between the expression levels of interleukin(IL)-33 and soluble growth stimulating expression gene 2 protein(sST2)in pleural effusion and inflammatory response.Methods A total of 108 patients with pleural effusion admitted to Hebei Provincial Chest Hospital from March 2019 to March 2022 were included in the study retrospectively.Patients with tuberculous pleural effusion(TPE)were included in the TPE group(n=45),and patients with malignant pleural effusion(MPE)were included in the MPE group(n=60).In the same period,50 healthy adults who underwent physical examination in our hospital were selected as the control group(n=50).The levels of IL-33 and sST2 in pleural fluid and serum IL-33,sST2,IL-6,C-reactive protein(CRP)and tumor necrosis factor-α(TNF-α)were detected.The correlation between pleural effusion,serum IL-33 and sST2 and serum inflammatory factors in patients with TPE and MPE was analyzed by Pearson correlation coefficient.The diagnostic value of pleural effusion and serum IL-33 and sST2 in TPE was analyzed by receiver operating characteristic(ROC)curve.Results The levels of IL-33 and sST2 in pleural effusion were(83.52±21.09),(1108.37±236.15)ng/L,respectively,which were higher in TPE group than those in MPE group[(53.16±11.24),(726.49±151.28)ng/L],the differences were statistically significant(P<0.05).The serum levels of IL-33 and sST2 in TPE group and MPE group were higher than those in control group,and TPE group was(74.13±18.49),(1454.07±477.19)ng/L,respectively,which was higher than that in MPE group[(58.46±16.52),(1228.65±374.64)ng/L],the differences were statistically significant(P<0.05).The levels of serum IL-6,CRP and TNF-αin TPE group and MPE group were higher than those in control group,and TPE group was higher than MPE group,the differences were statistically significant(P<0.05).Pleural effusion and serum IL-33 and sST2 were positively correlated with serum IL-6,CRP and TNF-αlevels in TPE patients(P<0.05).The pleural effusion and serum IL-33 a

关 键 词：结核性胸腔积液 恶性胸腔积液 白细胞介素-33 可溶性生长刺激表达基因2蛋白 炎症反应 

分 类 号：R561.3[医药卫生—呼吸系统]