他仑帕奈对脑缺血再灌注大鼠脑组织内Caspase-3表达及血清炎性因子的影响  

Effects of talampanel on Caspase-3 expression and serum inflammatory factors in brain tissue of rats with cerebral ischemia-reperfusion

作　　者：石秋艳 李冠一 杨斌 王翠兰 李弘 闫玉敏 何静远 Shi Qiuyan;Li Guanyi;Yang Bin(Neurology Intensive Care Unit,the Affiliated Hospital of North China University of Science and Technology,Tangshan 063000,China)

机构地区：[1]华北理工大学附属医院神经内科,河北唐山063000

出　　处：《华北理工大学学报（医学版）》2023年第3期191-199,共9页Journal of North China University of Science and Technology：Health Sciences Edition

基　　金：河北省2018年政府资助临床专科能力建设和专科带头人项目(编号:冀财社[2018]29)。

摘　　要：目的 通过观察大鼠脑缺血再灌注损伤(CIR)后脑组织内半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)及血清肿瘤坏死因子(TNF-a)、白细胞介素-1β(IL-1β)水平,探究他仑帕奈(TAL)对脑缺血再灌注后神经损伤的保护作用。方法 将90只SD大鼠分为假手术组、模型组及TAL治疗0h、1h、3h、5h组,每组各15只。通过线栓法构建大脑中动脉闭塞模型,依次在血流复灌注后0h、1h、3h和5h经尾静脉注射TAL(12mg/kg),24h后采用Longa评分法进行神经功能损伤评分,TTC染色测量脑梗死体积,免疫组化染色检测梗死周围区Caspase-3的表达及梗死皮质区神经元的存活情况,ELASA法检测血清TNF-a、IL-1β水平,综合评价TAL的神经保护作用。结果 与模型组及假手术组相比,TAL各治疗组大鼠脑梗死体积减小,神经功能损伤程度明显改善,海马CA1区神经元细胞凋亡减少,梗死周围区Caspase-3、血清TNF-α和IL-1β明显下降,且呈时间依赖性,差异均具有统计学意义(P<0.05)。结论 TAL能降低脑缺血再灌注区域Caspase-3的表达以及血清炎性因子的浓度,起到一定的神经保护作用,且早期应用效果更优,随时间延长,这种作用明显减弱。Objective To investigate the protective effects of tarentopanax on neurological injury after cerebral ischemia-reperfusion injury in rats by observing the levels of cysteine aspartate-3(Caspase-3)and serum inflammatory factors tumor necrosis factor(TNF-a)and interleukin-1β(IL-1β)in brain tissues after cerebral ischemia-reperfusion injury.Methods Ninety healthy SD male rats(weight 180g)were randomly divided into a sham-operated group(Sham group),right middle cerebral artery occlusion model group(MCAO group),and groups receiving Talampanel(TAL)treatment for 0h,1h,3h,and 5h,with 15 rats in each group.The rat middle cerebral artery occlusion model was formed by a modified Longa wire embolization method.After all rats were successfully anesthetized,In Sham group,only the right middle cerebral artery was isolated;The MCAO group ligated the right middle cerebral artery with sterile silk wire to make it completely occluded,and withdrew the wire after 1h to restore blood flow and establish a reperfusion model;The TAL group sequentially injected tarentopanax(12mg/kg)via tail vein at 0h,1h,3h and 5h after blood flow reperfusion.The rats in Sham and MCAO groups were given equal amount of saline through the tail vein.24h later,neurological impairment was scored in each group:the volume of cerebral infarction was measured by TTC staining,the expression of Caspase-3 in the peri-infarct area and the number of surviving neurons in the infarcted cortical area were detected by immunohistochemical staining,and the levels of serum TNF-a and IL-1βwere measured by ELASA method.Results Compared with the model group and sham operation group,the TAL treatment groups showed a decrease in the volume of cerebral infarction,a significant improvement in the degree of neurological damage,a decrease in neuronal cell apoptosis in the CA1 area of the hippocampus,and a decrease in Caspase 3 and serum TNF levels in the surrounding area of the TNF-a and IL-1β,there was a significant decrease and a time-dependent trend,the differences were statist

关 键 词：脑梗死 AMPA受体拮抗剂 他仑帕奈 缺血再灌注损伤 神经保护 

分 类 号：R96[医药卫生—药理学]