基于化学计量学宁夏银柴胡红外指纹图谱及伪品判别分析  

Infrared Fingerprint of Ningxia Stellarae Radix and Discriminant Analysis of Counterfeit Products Based on Chemometrics

作　　者：李振凯 马玲 白明生[1] 黄钰馨 宋乐 王红 冯璐 彭励[1] Li Zhenkai;Ma Ling;Bai Mingsheng;Huang Yuxin;Song Le;Wang Hong;Feng Lu;Peng Li(School of Life Sciences,Ningxia University,Yinchuan 750021,China;Ningxia Hui Autonomous Region Institute of Drug Control,Yinchuan 750002,China)

机构地区：[1]宁夏大学生命科学学院,银川750021 [2]宁夏回族自治区药品检验研究院,银川750002

出　　处：《世界科学技术-中医药现代化》2023年第1期175-183,共9页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：宁夏回族自治区自然科学基金委员会一般项目(2021AAC03103):宁夏道地药材银柴胡代谢物特征及其与产地生境因子相关性研究,负责人:彭励;同心县科学技术局科技发展计划项目(201901)“:同心银柴胡”道地性研究,负责人:彭励。

摘　　要：目的建立宁夏回族自治区银柴胡其及伪品的中红外、近红外光谱指纹图谱,并结合化学计量学分析比较两种光谱技术在银柴胡质量评价、真伪鉴定上的应用效果。方法分别利用傅里叶红外光谱仪和傅里叶变换近红外光谱仪,采集不同来源宁夏回族自治区银柴胡及伪品的中红外和近红外光谱图谱,借助Origin 2018软件对图谱进行预处理、图谱合成以及相似度评价、系统聚类分析等化学计量学分析。结果初步确定了27个银柴胡中红外图谱共有特征峰和11个近红外图谱共有特征峰;两种光谱图谱经二阶导数等预处理后,结合2种化学计量学分析方法均可以鉴别银柴胡伪品,其中近红外光谱结合系统聚类分析的效果最为显著,并可以有效区分不同来源银柴胡药材。结论近红外光谱结合化学计量学分析在银柴胡药材真伪优劣判别中的应用效果更好,且更符合实际生产需求。研究结果对建立快速的道地银柴胡溯源、真伪判别方法,进一步完善质量评价体系有积极的借鉴意义。Objective To establish the mid-infrared and near-infrared spectral fingerprints of Stellarae Radix and its adulterants in Ningxia,and to compare the application effects of the two spectral techniques in quality evaluation and authenticity identification of Stellarae Radix.Methods The mid-infrared and near-infrared spectra of Stellarae Radix and its adulterants from different sources were collected by Fourier transform infrared spectrometer and Fourier transform near-infrared spectrometer respectively,and the chemometrics analysis was carried out with the help of Origin 2018 software,such as pretreatment,map synthesis,similarity evaluation and systematic cluster analysis.Results The common characteristic peaks of 27 middle infrared spectra and 11 near infrared spectra of Stellarae Radix were preliminarily determined.After the two spectra were pretreated by second derivative,combined with two chemometrics analysis methods,the counterfeit products of Stellarae Radix could be identified,among which the effect of near infrared spectroscopy combined with systematic cluster analysis was the most significant,and different sources of Stellarae Radix can be distinguished.Conclusion The application effect of near-infrared spectroscopy combined with chemometrics analysis in judging the authenticity of Stellarae Radix is better,and it is more in line with the actual production requirements.The research results have positive reference significance for establishing a rapid and accurate discrimination method of Stellarae Radix and further improving the quality evaluation system.

关 键 词：银柴胡 中红外 近红外 指纹图谱 化学计量学 质量评价 真伪鉴别 

分 类 号：R284.1[医药卫生—中药学]