Synthesis of easily-processable collagen bio-inks using ionic liquid for 3D bioprinted liver tissue models with branched vascular networks  

作　　者：Zhiqiang Gao Xin Liu Hao Zhao Shengpeng Xia Wenli Liu Haotian Bai Fengting Lv Xiongfei Zheng Yiming Huang Qi Gu Shu Wang 

机构地区：[1]Beijing National Laboratory for Molecular Sciences,Key Laboratory of Organic Solids,Institute of Chemistry,Chinese Academy of Sciences,Beijing 100190,China [2]State Key Laboratory of Membrane Biology,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China [3]Beijing Institute for Stem Cell and Regenerative Medicine,Beijing 100101,China [4]College of Chemistry,University of Chinese Academy of Sciences,Beijing 100049,China [5]Shenyang Institute of Automation,Chinese Academy of Sciences,Shenyang 110169,China

出　　处：《Science China Chemistry》2023年第5期1489-1499,共11页中国科学（化学英文版）

基　　金：supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA16020804,XDA16020802);the National Natural Science Foundation of China(22021002,22022705)。

摘　　要：Bioprinting has been a flouring way to fabricate complex tissue and organ mimics via precisely depositing printable cell-laden biomaterials.However,there is a limited number of biomaterials that fulfill the mechanical property of printing while meeting the responsive environment desired for the cells.Despite excellent cell compatibility and bioactivity,collagen suffers from difficulties in processing and printability which inhibited its utilization in three-dimensional(3D)bioprinting.Herein,we address this limitation by using ionic liquid as the solvent in the modification process,enabling collagens modified with quantified norbornene for chemical crosslink and extrusion-based 3D printing.With improved solubility and rheological properties,norbornene-functionalized collagen(Col-Nor)exhibited better shape fidelity in extrusion-based 3D printing compared with the one before modification.Photo-crosslinked Col-Nor hydrogel provided structural support and promoted the adhesion,proliferation,and differentiation of various types of cells,which afforded a centimeter-scale liver tissue model.This highly generalizable methodology expands printable,versatile,and tunable hydrogels developed from the natural extracellular matrix,allowing the biofabrication of 3D liver tissue model with branched vascular networks.

关 键 词：BIOPRINTING functionalized collagen rheological properties PHOTO-CROSSLINKING 3D tissue model 

分 类 号：R318[医药卫生—生物医学工程] TQ427.26[医药卫生—基础医学]