Birth-and-death evolution of ribonuclease 9 genes in Cetartiodactyla  

作　　者：Datian Lang Xiaoping Wang Chunbing Liu Weihang Geng David M.Irwin Shanyuan Chen Chunqing Li Li Yu Heng Xiao 

机构地区：[1]School of Life Sciences,Yunnan University,Kunming 650500,China [2]School of Ecology and Environmental Science,Yunnan University,Kunming 650091,China [3]State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan,Yunnan University,Kunming 650091,China [4]Biodiversity Research Center of Wumeng Mountain,Department of Agronomy and Life Science,Zhaotong University,Zhaotong 657000,China [5]Department of Laboratory Medicine and Pathobiology,University of Toronto,Toronto,Ontario M5S 1A8,Canada

出　　处：《Science China(Life Sciences)》2023年第5期1170-1182,共13页中国科学（生命科学英文版）

基　　金：supported by the Special Basic Cooperative Research Programs of Yunnan Provincial Undergraduate Universities(202001AO070195);the National Natural Science Foundation of China(31925006);the Applied Basic Research General Project of Yunnan Science Technology Department(202001BB050058)。

摘　　要：RNase9 plays a reproductive function and has been recognized as an important member of the ribonuclease(RNase)A superfamily,a gene family that is widely used as a model for molecular evolutionary studies.Here,we identified 178 RNase9 genes from 95 Cetartiodactyla species that represent all four lineages and 21 families of this clade.Unexpectedly,RNase9experienced an evolutionary scenario of“birth and death”in Ruminantia,and expression analyses showed that duplicated RNase9A and RNase9B genes are expressed in reproductive tissues(epididymis,vas deferens or prostate).This expression pattern combined with the estimate that these genes duplicated during the middle Eocene,a time when Ruminantia become a successful lineage,suggests that the RNase9 gene duplication might have been advantageous for promoting sperm motility and male fertility as an adaptation to climate seasonality changes of this period.In contrast,all RNase9 genes were lost in the Cetacean lineage,which might be associated with their high levels of prostatic lesions and lower reproductive rates as adaptations to a fully aquatic environment and a balance to the demands of ocean resources.This study reveals a complex and intriguing evolutionary history and functional divergence for RNase9 in Cetartiodactyla,providing new insights into the evolution of the RNaseA superfamily and molecular mechanisms for organismal adaptations to the environment.

关 键 词：RNase9 gene duplication gene loss CETARTIODACTYLA 

分 类 号：Q75[生物学—分子生物学]