纯钽表面微弧氧化“类骨小梁”状多孔涂层的细胞相容性  

Cytocompatibility of"Trabecular Bone-like"Porous Coating Prepared by Micro-arc Oxidation on Pure Tantalum

作　　者：王伟强[1] 王舒月 于凤云[1] 王轶农[1] WANG Wei-qiang;WANG Shu-yue;YU Feng-yun;WANG Yi-nong(Dalian University of Technology,Liaoning Dalian 116024,China)

机构地区：[1]大连理工大学,辽宁大连116024

出　　处：《表面技术》2023年第4期363-373,共11页Surface Technology

摘　　要：目的 提高医用纯钽的生物活性,利用微弧氧化(MAO)技术在其表面制备出“类骨小梁”状分级多孔涂层,并对比该涂层与传统“火山坑”状MAO涂层以及机械抛光纯钽表面在表面粗糙度、亲水性以及细胞相容性方面的差异。方法 使用0.1 mol/L Na2B4O7和0.05 mol/L Na3PO4电解液在纯钽表面分别制备出“类骨小梁”状及“火山坑”状多孔涂层(分别命名为B-MAO和P-MAO涂层)。采用扫描电镜、X射线衍射以及X射线光电子能谱对不同结构涂层进行形貌观察和相组成分析,使用十字划格法评价涂层结合强度,使用激光共聚焦显微镜测定涂层的表面粗糙度,使用接触角仪测量其亲水性,并将小鼠前成骨细胞(MC3T3-E1)接种于材料表面,对比不同形貌状态对细胞铺展、增殖以及成骨分化的影响。结果 MAO涂层物相主要为Ta2O5。B-MAO涂层由于内部孔隙度高,应力释放充分,涂层结合强度高,而P-MAO涂层则因具有分层现象和较大的残余应力,易从基体剥落。抛光纯钽表面、P-MAO、B-MAO涂层表面的粗糙度分别为0.06、1.50、1.58μm,与之相应的接触角分别为42.6°、15.5°、7.2°。细胞初期粘附结果表明,MAO涂层表面细胞数量多于抛光纯钽表面,且B-MAO涂层细胞铺展能力优于P-MAO涂层和抛光钽表面。CCK-8测试结果表明,细胞数量随时间逐渐增加,MAO组细胞增殖能力好于抛光组,B-MAO组细胞增殖能力最优。ALP活性方面,B-MAO组也高于P-MAO和抛光组。结论 MAO涂层相较于抛光纯钽表面具有更高的粗糙度以及亲水性,从而具有高的细胞粘附和增殖能力。“类骨小梁”状分级多孔涂层由于具有更高的结合强度和超亲水性,在细胞铺展、增殖以及成骨分化能力高于“火山坑”状单级多孔涂层,在硬组织替代领域具有较大应用潜力。Tantalum is widely used as a biomedical implant material,but the bioinert of its surface may affect its bonding with bone tissue.To improve the bioactivity of tantalum,researchers usually treat it with surface modification,among which micro-arc oxidation(MAO)is an effective method.The MAO can prepare a ceramic coating with a porous structure and high coating adhesion.Meanwhile,it can easily incorporate some bioactive elements into the coating.In this work,a novel"trabecular bone-like"hierarchical porous coating was prepared on the tantalum surface by MAO to improve its bioactivity.The difference between this coating and the conventional"crater-like"MAO coating as well as the mechanically polished pure tantalum surface was compared in terms of surface roughness,hydrophilicity,cytocompatibility,etc.A tantalum rod(>99.9 wt%)with a diameter of 10 mm was cut into tantalum sheets with a thickness of 2 mm.All tantalum sheets were polished with emery paper and cleaned in an ultrasonic cleaner.The MAO was conducted with a bipolar pulse power supply with a constant current model.The frequency and duty circle were set at 600 Hz and 9%,respectively.The"trabecular bone-like"porous coatings(appointed as B-MAO coatings)were prepared on tantalum sheets with 0.1 M Na2B4O7 electrolyte with the work current set to 4 A.The"crater-like"porous coatings(appointed as P-MAO coatings)were prepared on tantalum sheets with 0.05 M Na3PO4 electrolyte with the work current set to 12 A.Scanning electron microscopy(SEM),X-ray diffraction(XRD),and X-ray photoelectron spectroscopy(XPS)were used to observe the coating's morphology and to analyze the coating's phase composition.The coating adhesion was evaluated through the cross-scratch method.Their surface roughness was analyzed with confocal laser scanning microscopy(CLSM).The surface hydrophilicity was measured with a contact angle(CA)meter.And mouse preosteoblasts(MC3T3-E1)were seeded on the surface of the materials to compare the effects of different surface morphology on cell spreading,prolif

关 键 词：钽 微弧氧化 四硼酸钠 多孔涂层 亲水性 细胞相容性 

分 类 号：TB34[一般工业技术—材料科学与工程]