微管调节试剂对白菜型冬油菜萌动种子内源激素及抗寒基因表达的影响  

Effects of microtubule reagent treatment on endogenous hormone and cold-tolerance related genes of seedling winter rapeseed Brassica rapa L.

作　　者：田海燕 冯秀英 徐明霞 董小云 郑国强 李辉 王莹 魏家萍 武泽峰 崔俊美 谭雪莲[3] 刘自刚 TIAN Haiyan;FENG Xiuying;XU Mingxia;DONG Xiaoyun;ZHENG Guoqiang;LI Hui;WANG Ying;WEI Jiaping;WU Zefeng;CUI Junmei;TAN Xuelian;LIU Zigang(College of Agronomy,Gansu Agricultural University/State Key Laboratory of Aridland Crop Science/Gansu Key Lab.of Crop Improvement&Germplasm Enhancement,Lanzhou,Gansu 730070,China;Wushan County Agricultural Technology Extension Center,Tianshui,Gansu 741300,China;Institute of Dryland Agriculture,Gansu Academy of Agricultural Sciences,Lanzhou,Gansu 730070,China)

机构地区：[1]甘肃农业大学农学院/甘肃省干旱生境作物学重点实验室/甘肃省作物遗传改良与种质创新重点实验室,甘肃兰州730070 [2]武山县农业技术推广中心,甘肃天水741300 [3]甘肃省农业科学院旱地农业研究所,甘肃兰州730070

出　　处：《干旱地区农业研究》2023年第3期63-70,共8页Agricultural Research in the Arid Areas

基　　金：甘肃省科技重大专项(22ZD6NA009);省部共建干旱生境作物学国家重点实验室主任基金(GSCS-2021-Z01);甘肃省教育厅产业支撑计划项目(2021CYZC-46);甘肃省教育厅青年博士基金项目(2021QB-035);中央引导地方科技专项(ZCYD2020-2-3);西藏自治区农科院农科所统筹基金项目(2021NKS-TCJJ-001);甘肃省教育厅2023年研究生“创新之星”项目(2023CXZX-647)。

摘　　要：以‘陇油7号’和‘天油4号’2个抗寒性不同的白菜型冬油菜为试验材料,通过添加400μmol·L-1秋水仙碱(微管聚合抑制剂)和50μmol·L-1紫杉醇(微管聚合促进剂)试剂后,在20℃和0℃下分别处理12 h和24 h,随后采用液相色谱质谱联用仪和荧光定量PCR测定萌动种子内源激素含量及抗寒基因表达量,研究微管调节试剂对白菜型冬油菜萌动种子内源激素和抗寒基因的影响。结果表明:与对照相比,添加秋水仙碱后,在0℃和20℃处理下‘陇油7号’和‘天油4号’ABA、IAA、GA 3、ZT含量明显降低;添加紫杉醇后,‘陇油7号’和‘天油4号’ABA、IAA、GA 3、ZT含量明显升高。添加秋水仙碱后,与0℃处理12 h相比,0℃处理24 h时,‘陇油7号’α-tubulin、β-tubulin、MKK2、ICE1、CBF1、BrAFP1的表达量增加,‘天油4号’α-tubulin、β-tubulin、CBF1表达量增加,但MKK2、ICE1、BrAFP1的表达量降低;与20℃处理12 h相比,20℃处理24 h时,‘陇油7号’和‘天油4号’α-tubulin、β-tubulin、MKK2、ICE1、CBF1、BrAFP1的表达量均增加。添加紫杉醇后,与0℃处理12 h相比,0℃处理24 h时,‘陇油7号’α-tubulin、β-tubulin、ICE1、CBF1、BrAFP1的表达量均降低,‘天油4号’α-tubulin、β-tubulin、ICE1、CBF1、BrAFP1的表达量增加;与20℃处理12 h相比,20℃处理24 h时,‘陇油7号’和‘天油4号’α-tubulin、β-tubulin、MKK2、ICE1、CBF1、BrAFP1的表达量均降低。由此可知,微管调节试剂会影响白菜型冬油菜萌动种子内源激素的含量及抗寒基因的表达。In this study,‘Longyou 7’and‘Tianyou 4’were treated with 400μmol·L-1 colchicine(Col)and 50μmol·L-1 taxol(Tax)for 12 h and 24 h at 20℃and 0℃and followed by the sample liquid chromatography and quantitative PCR to determine the effects of microtubule reagent on endogenous hormones and cold-tolerance related genes in seedling winter rapeseed.The results showed that the contents of‘Longyou 7’and‘Tianyou 4’,ABA,IAA,GA 3 and ZT decreased significantly after adding Col,and the levels of ABA,IAA,GA 3 and ZT increased significantly after Tax.After adding Col,compared to the 0℃treatment for 12 h,when the 0℃was processed for 24 h,the expressions of‘Longyou 7’α-tubulin,β-tubulin,MKK2,ICE1,CBF1,and BrAFP1 were upregulated,‘Tianyou 4’α-tubulin,β-tubulin and CBF1 were upregulated,however,the expression level of MKK2,ICE1 and BrAFP1 was downregulated;compared to the 20℃treatment for 12 h,at 20℃treatment for 24 h,the expressions of‘Longyou 7’and‘Tianyou 4’α-tubulin,β-tubulin,MKK2,ICE1,CBF1,and BrAFP1 were upregulated.After adding Tax,compared to the 0℃treatment for 12 h,when the 0℃was processed for 24 h,the expressions of‘Longyou 7’α-tubulin,β-tubulin,ICE1,CBF1 and BrAFP1 were downregulated.The expression of‘Tianyou 4’α-tubulin,β-tubulin,ICE 1,CBF1 and BrAFP1 was upregulated;compared to the 20℃treatment for 12 h,at 20℃treatment for 24 h,the expressions of‘Longyou 7’and‘Tianyou 4’α-tubulin,β-tubulin,MKK2,ICE1,CBF1,and BrAFP1 were downregulated.This showed that microtubule regulating reagent affected the endogenous hormone content and the expression of cold resistance genes in cabbage winter rape.

关 键 词：白菜型冬油菜 萌动种子 低温 内源激素 抗寒基因表达 秋水仙碱 紫杉醇 

分 类 号：S565.4[农业科学—作物学]