ZnO@Au的制备及在免分离荧光测定microRNA-21中的应用  

Preparation of ZnO@Au and its application in the separation free fluorescence determination of microRNA-21

作　　者：高帆 阙永辉 许永涛 陈德伦[2] 李易 王小红[3] GAO Fan;QUE Yong-hui;XU Yong-tao;CHEN De-lun;LI Yi;WANG Xiao-hong(College of Chemical Engineering and Technology,Hainan University,Haikou 570228,China;College of Materials Science and Engineering,Hainan University,Haikou 570228,China;College of Science,Hainan University,Haikou 570228,China)

机构地区：[1]海南大学化学工程与技术学院,海南海口570228 [2]海南大学材料科学与工程学院,海南海口570228 [3]海南大学理学院,海南海口570228

出　　处：《化学研究与应用》2023年第5期1210-1216,共7页Chemical Research and Application

基　　金：海南省基础与应用基础研究计划(自然科学领域)高层次人才项目(2019RC114)资助。

摘　　要：本论文利用水热法与原位生长策略,在FTO表面制备了多孔ZnO@Au复合纳米材料,并在其表面修饰捕获探针以特异性识别肿瘤标志物microRNA-21。借助于外切酶ExoⅢ对双链的剪切作用,吸附有目标RNA的捕获探针被剪切从而释放出荧光基团,造成荧光信号的显著增强,以此实现microRNA-21的荧光传感。结果表明,所制备的ZnO@Au复合材料及传感策略有效地避免了荧光分析中分离步骤可能出现的假阳性现象,在1 nM-500 nM浓度范围内具有良好的线性关系,检测限低至1 nM。In this paper,porous ZnO@Au composite nanomaterials were prepared on the surface of FTO using hydrothermal method and in situ growth strategy,and the surface was modified with capturing probes to specifically identify tumor marker microRNA-21.With the help of ExoⅢ's shear action on the double strand,the capture probe adsorbent on the target RNA is shear,thus releasing the fluorescence group,resulting in a significant enhancement of the fluorescence signal,so as to realize the fluorescence sensing of microRNA-21.The results show that the ZnO@Au composite and its sensing strategy can effectively avoid the false positive phenomenon that may occur in the separation step of fluorescence analysis,and have a good linear relationship in the concentration range of 1 nm-500 nM,and the detection limit is as low as 1 nM.

关 键 词：MICRORNA-21 荧光检测 氧化锌纳米材料 金纳米粒子 

分 类 号：O657.3[理学—分析化学]