银胶菊中木脂素类成分的研究  

Lignans from Parthenium hysterophorus Linn

作　　者：李华 王素娟 李红霞 张贵杰[1] LI Hua;WANG Su-juan;LI Hong-xia;ZHANG Gui-jie(College of Pharmacy,Guilin Medical University,Guilin 541199,China)

机构地区：[1]桂林医学院药学院,桂林541199

出　　处：《天然产物研究与开发》2023年第5期761-765,780,共6页Natural Product Research and Development

基　　金：国家自然科学基金(21967007)。

摘　　要：研究银胶菊Parthenium hysterophorus Linn.中木脂素类化学成分。采用硅胶、Sephadex LH-20、MCI、ODS和半制备型HPLC等色谱技术,从银胶菊地上部分的95%乙醇提取物中分离得到5个木脂素类化合物(1~5),通过波谱学数据分析,将它们的结构分别鉴定为(7S,8S,7′E)-4,9,9′-三羟基-3,5,7,3′,5′-五甲氧基-8,4′-氧新木脂素-7′-烯(1)、丁香脂素(2)、杜仲树脂酚(3)、松脂素(4)、五味子醇甲(5)。其中,化合物1为新化合物,化合物3~5为首次从银胶菊中分离得到。对所有化合物进行了体外抗神经炎症活性筛选,结果显示,化合物1对LPS激活的BV-2小胶质细胞释放NO具有显著抑制作用,IC_(50)为21.10±1.97μmol/L,其余化合物均无活性,且所有化合物在测试浓度下均不影响细胞的存活率。To study the lignans from Parthenium hysterophorus Linn.Five lignans(1-5)were isolated from the 95%ethanol extract of the aerial parts of P.hysterophorus by silica gel,Sephadex LH-20,MCI,ODS,and preparative HPLC chromatographic techniques.Their structures were established on the basis of extensive spectroscopic analysis and comparison with the data in reported literatures.As a result,five compounds were identified as(7 S,8 S,7′E)-4,9,9′-trihydroxy-3,5,7,3′,5′-pentamethoxy-8,4′-oxyneolign-7′-ene(1),syringaresinol(2),medioresinol(3),pinoresinol(4),schisandrin(5).Compound 1 was a new compound,and compounds 3-5 were isolated from P.hysterophorus for the first time.All the compounds were screened for their in vitro anti-neuroinflammatory activities,and the results showed that compound 1 displayed significant activity with an IC 50 value of 21.10±1.97μmol/L.The other compounds were inactive,and all the compounds didn′t affect the cell survival under the test concentrations.

关 键 词：银胶菊 木脂素 小胶质细胞 抗神经炎症活性 

分 类 号：R284.2[医药卫生—中药学]