鸡蛋果SUT基因家族的鉴定及表达分析  

Identification and expression analysis on SUT gene family of Passiflora edulis

作　　者：李韵 刘昱君 潘若云 魏秀清[1,2,3] 曾黎辉[1,2] 方庭 LI Yun;LIU Yujun;PAN Ruoyun;WEI Xiuqing;ZENG Lihui;FANG Ting(Fujian Agriculture and Forestry UniversityCollege of Horticulture,Fuzhou 350002,China;Fujian Agriculture and Forestry UniversityInstitute of Horticultural Plant Genetics and Breeding,Fuzhou 350002,China;Fruit Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou 350013,China)

机构地区：[1]福建农林大学园艺学院,福建福州350002 [2]福建农林大学园艺植物遗传育种研究所,福建福州350002 [3]福建省农业科学院果树研究所,福建福州350013

出　　处：《植物资源与环境学报》2023年第3期33-44,共12页Journal of Plant Resources and Environment

基　　金：福建省自然科学基金项目(2021J05022);福建省种业创新与产业化工程项目(zycxny2021010);福建省高原学科建设经费(102/71201801101);福建省公益类科研院所专项(2019R1028-9)。

摘　　要：采用生物信息学方法,从鸡蛋果(Passiflora edulis Sims)全基因组中鉴定SUT基因家族成员,并对其编码蛋白的理化性质、亚细胞定位、三级结构和系统发育以及基因结构、共线性和表达模式进行了分析。结果显示:鸡蛋果全基因组中鉴定出的5个PeSUT基因定位在4条染色体上,绝大多数SUT基因家族成员含有8或9个外显子,其编码蛋白的氨基酸残基数介于328~1014之间,均定位于质膜。鸡蛋果5个SUT家族成员分为3个亚族。鸡蛋果SUT基因家族中PeSUT 1和PeSUT 5基因以及PeSUT 2和PeSUT 3基因存在共线关系。鸡蛋果3个SUT基因与拟南芥〔Arabidopsis thaliana(Linn.)Heynh.〕4个SUC基因间存在共线关系。鸡蛋果SUT基因家族启动子中存在大量与激素和逆境胁迫响应相关的顺式作用元件。转录组数据分析结果表明:PeSUT 1和PeSUT 2基因在鸡蛋果品种‘台农1号’(‘Tainong No.1’)和‘黄金果’(‘Huangjinguo’)叶片和果皮中表达量较低或不表达;PeSUT 3基因在‘台农1号’叶片中的表达量低于‘黄金果’叶片,但在‘台农1号’果皮中的表达量却高于‘黄金果’果皮;PeSUT 4基因在‘台农1号’叶片和果皮中的表达量分别高于‘黄金果’叶片和果皮;PeSUT 5基因在‘台农1号’和‘黄金果’叶片中的表达量基本相同,在‘台农1号’果皮中的表达量低于‘黄金果’果皮。实时荧光定量PCR分析结果表明:PeSUT 3基因在低温(4℃)胁迫处理下的相对表达量较正常温度(25℃)处理极显著(P≤0.01)上调。综合分析结果表明:PeSUT 3基因可能参与鸡蛋果叶片对低温胁迫的响应。The SUT gene family members were identified from whole genome of Passiflora edulis Sims by using bioinformatics methods,and physicochemical properties,subcellular localization,tertiary structure,and phylogeny of their encoded proteins,gene structure,collinearity,and expression pattern were analyzed.The results show that five PeSUT genes identified from whole genome of P.edulis are located on four chromosomes,most SUT gene family members contain eight or nine exons,the number of amino acid residues of their encoded proteins are within 328-1014,and they are all located on plasma membrane.Five SUT family members of P.edulis are divided into three sub-families.There are collinear relationships between PeSUT 1 and PeSUT 5 genes and between PeSUT 2 and PeSUT 3 genes in SUT gene family of P.edulis.There are collinear relationships between three SUT genes of P.edulis and four SUC genes of Arabidopsis thaliana(Linn.)Heynh.There are a large number of cis-acting elements related with hormone and stress response in promoters of SUT gene family of P.edulis.The transcriptomic data analysis result shows that the expression of PeSUT 1 and PeSUT 2 genes in leaves and pericarps of cultivar‘Tainong No.1’and‘Huangjinguo’of P.edulis is low or not expressed;the expression of PeSUT 3 gene in leaves of‘Tainong No.1’is lower than that in leaves of‘Huangjinguo’,but is higher in pericarps of‘Tainong No.1’than that in pericarps of‘Huangjinguo’;the expression of PeSUT 4 gene in leaves and pericarps of‘Tainong No.1’is higher than those in leaves and pericarps of‘Huangjinguo’,respectively;the expression of PeSUT 5 gene in leaves of‘Tainong No.1’and‘Huangjinguo’is basically the same,but the expression in pericarps of‘Tainong No.1’is lower than that in pericarps of‘Huangjinguo’.The real-time fluorescence quantitative PCR analysis result shows that the relative expression of PeSUT 3 gene under low temperature(4℃)stress treatment is extremely significantly(P≤0.01)up-regulated compared with that u

关 键 词：鸡蛋果 蔗糖转运蛋白(SUTs) 基因家族 表达 低温胁迫 

分 类 号：Q943.2[生物学—植物学] S667.9[农业科学—果树学]