不同葡萄品种的耐盐性比较分析  

Effects of NaCl stress on antioxidant enzyme content and malondialdehyde content in grape cultivars leaves

作　　者：陈丽靓 鲁倩君 马媛媛 刘迎 赵宝龙[1] 孙军利[1] CHEN Liliang;LU Qianjun;MA Yuanyuan;LIU Ying;ZHAO Baolong;SUN Junli(Key Laboratory of Characteristic Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization of Xinjiang Production and Construction Corps/College of Agronomy,Shihezi University,Shihezi Xinjiang 832003,China)

机构地区：[1]石河子大学农学院/特色果蔬栽培生理与种质资源利用兵团重点实验室,新疆石河子832000

出　　处：《新疆农业科学》2023年第4期880-888,共9页Xinjiang Agricultural Sciences

基　　金：国家自然科学基金项目“葡萄抗盐砧木筛选及其耐盐适应性反应机制研究”(32060647);国家自然科学基金项目“葡萄抗性砧木提高赤霞珠植株白藜芦醇含量的机理研究”(32060648);石河子大学育种项目(XZZX202104)。

摘　　要：【目的】研究不同葡萄品种耐盐性,为耐盐葡萄品种的筛选提供理论支持。【方法】以葡萄品种5BB、101-14、SO4、3309M、贝达、抗砧3号、喀什哈尔、木纳格、和田红、夏黑10份盆栽葡萄品种幼苗为材料,采用盆栽灌盐培养的方式,研究100 mmol/L NaCl胁迫对不同葡萄品种叶片的盐害指数、光合作用、抗氧化酶含量和丙二醛含量等指标的影响,运用主成分分析比较其耐盐性。【结果】100 mmol/L NaCl胁迫下,和田红、木纳格、喀什哈尔、夏黑的SOD、CAT、POD活性、净光合速率(Pn)、气孔导度(Gs)和蒸腾速率(Tr)与对照相比增幅最大,胞间二氧化碳(Ci)和丙二醛含量降幅最小;101-14、抗砧3号、贝达的SOD、CAT、POD活性、净光合速率(Pn)、气孔导度(Gs)和蒸腾速率(Tr)与对照相比增幅较大,胞间二氧化碳(Ci)和丙二醛含量降幅较小;3309M、5BB、S04的SOD、CAT、POD活性,净光合速率(Pn)、气孔导度(Gs)和蒸腾速率(Tr)与对照相比增幅最小,胞间二氧化碳(Ci)和丙二醛含量降幅最小。【结论】和田红、木纳格、喀什哈尔、夏黑为耐盐品种,101-14、抗砧3号、贝达为中等耐盐品种,3309 M、5BB、S04为不耐盐品种。【Objective】To compare grape cultivars salt tolerance in the hope of providing theoretical support for the selection of salt-tolerant grape varieties by using ten grape varieties.【Methods】The experimental materials were cultured by pot irrigation with salt by adopting potted salt irrigation method cultivate to study the effects of 100 mmol/L NaCl stress on the salt damage index,photosynthesis,antioxidant enzyme content and malondialdehyde content of different grape varieties.Meanwhile,salt tolerance was compared through principal component analysis.【Results】Under 100 mmol/L NaCl stress,the activities of SOD,CAT,POD,net photosynthetic rate(Pn),stomatal conductance(Gs)and transpiration of Kashhar,Munage,Hetian Red,Summer Black the rate(Tr)had the largest increase compared to the control.The content of intercellular carbon dioxide(Ci)and malondialdehyde decreased the least.The SOD,CAT,POD activities,net photosynthetic rate(Pn),stomatal conductance(Gs)and transpiration rate(Tr)of 101-14,Beda,Kangzhen 3 increased significantly compared with the control.The content of intercellular carbon dioxide(Ci)and malondialdehyde decreased slightly.The SOD,CAT,POD activities,net photosynthetic rate(Pn),stomatal conductance(Gs)and transpiration rate(Tr)of 5BB,SO4,3309M had the smallest increase compared with the control,and the increase of intercellular carbon dioxide(Ci)and The content of malondialdehyde decreased the least.【Conclusion】Kashhar,Munage,Hetian Red and Summer Black are salt-tolerant varieties,101-14,Beda and Kangzhen 3 are moderately salt-tolerant varieties,and 5BB,SO4 and 3309M are salt intolerant varieties.

关 键 词：葡萄 NACL胁迫 耐盐性 光合作用 抗氧化酶 丙二醛 

分 类 号：S663.1[农业科学—果树学]