建昌帮炆法对远志4种成分在大鼠体内药代动力学和组织分布的影响研究  

Effect of Jianchang stewing method on the pharmacokinetics and tissue distribution of 4 components in Polygala tenuifolia in rats

作　　者：熊晓莉 陆美霞 施林峰 钟凌云[1] 叶喜德[1] XIONG Xiao-li;LU Mei-xia;SHI Lin-feng;ZHONG Ling-yun;YE Xi-de(School of Pharmacy,Jiangxi University of Chinese Medicine,Nanchang 330004)

机构地区：[1]江西中医药大学药学院,南昌330004

出　　处：《中南药学》2023年第5期1149-1156,共8页Central South Pharmacy

基　　金：国家重点研发计划中医药现代化研究重点专项(No.2018YFC1707205);江西省中医药中青年骨干人才(第一批)培养计划【赣中医药科教字〔2020〕2号】;中药炮制技术传承创新团队(No.CXTD22003)。

摘　　要：目的研究建昌帮炆法对远志中4种寡糖酯类成分在大鼠体内药代动力学和组织分布作用的影响。方法以炆远志为研究对象,生远志为对照,运用UPLC-MS/MS法测定远志炮制前后大鼠血浆和组织中西伯利亚远志糖A5、西伯利亚远志糖A6、细叶远志苷A、3,6'-二芥子酰基蔗糖的浓度。选取灌胃给药后13个时间点的血浆样品和4个时间点的组织样品,测定大鼠血浆和组织中4种寡糖酯类成分的浓度,并运用DAS 3.2.1软件计算药代动力学参数,SPSS 21.0分析主要参数的差异,比较两组血药浓度和组织浓度的差异。结果与生远志组相比,炆远志组西伯利亚远志糖A5、西伯利亚远志糖A6、3,6'-二芥子酰基蔗糖的Cmax和AUC0~12均明显增加(P<0.05),细叶远志苷A的Cmax和AUC0~12虽差异无统计学意义,但具有增加趋势,并在6个组织中浓度整体均增加。结论远志经炆制后,有利于4种寡糖酯类成分的吸收和分布,可提高其生物利用率。Objective To determine the effect of Jianchang stewing method on the pharmacokinetics and tissue distribution of 4 oligosaccharide esters in Polygala tenuifolia in rats.Methods Licoricesimmered Polygala tenuifolia was used as the study object,Polygala tenuifolia as the control,the concentrations of sibiricose A5,sibiricose A6,tenuifoliside A and 3,6'-disinapoyl sucrose in the plasma and tissue before and after the processing were determined by UPLC-MS/MS.The plasma samples at 13 time points and tissue samples at 4 time points after the intragastric administration were selected to determine the concentrations of 4 oligosaccharide esters in the rat plasma and tissue.The pharmacokinetic parameters were calculated by DAS 3.2.1 software.SPSS 21.0 was used to analyze the differences in the main parameters to compare the blood concentration and tissue concentration of both groups.Results Compared with the Polygala tenuifolia group,Cmax and AUC0~12 of sibiricose A5,sibiricose A6,and 3,6'-disinapoyl sucrose were significantly increased in Licorice-simmered Polygala tenuifolia group(P<0.05).There was no significant difference in Cmax and AUC0~12 of tenuifoliside A,and the concentrations of the 4 components increased in 6 tissues as a whole.Conclusion The stewing of Polygala tenuifolia is conducive to the absorption and distribution of the above 4 oligosaccharide esters and improving their bioavailability.

关 键 词：生远志 炆远志 炮制 药代动力学 组织分布 

分 类 号：R284.1[医药卫生—中药学] R285[医药卫生—中医学]