小鼠神门穴与心脏的逆向跨突触病毒示踪研究  

Study on reverse transsynaptic virus tracing targeting Shenmen(HT7)and heart in mice

作　　者：吴立斌 张帆 余情 刘磊 魏霞 蔡荣林 吴子建 胡玲 WU Libin;ZHANG Fan;YU Qing;LIU Lei;WEI Xia;CAI Ronglin;WU Zijian;HU Ling(Postgraduate School,Anhui University of Chinese Medicine,Hefei 230038,China;Anhui Key Laboratory of Acupuncture-moxibustion Basis and Technology,Hefei 230038,China;Research Institute of Acupuncture and Moxibustion,Anhui Academy of Chinese Medicine,Hefei 230038,China)

机构地区：[1]Postgraduate School,Anhui University of Chinese Medicine,Hefei 230038,China [2]Anhui Key Laboratory of Acupuncture-moxibustion Basis and Technology,Hefei 230038,China [3]Research Institute of Acupuncture and Moxibustion,Anhui Academy of Chinese Medicine,Hefei 230038,China

出　　处：《Journal of Acupuncture and Tuina Science》2023年第2期109-120,共12页针灸推拿医学（英文版）

基　　金：国家自然科学基金项目,No.81774414,No.81973757,No.82074536,No.82104999;安徽省自然科学基金项目,No.2108085QH364,No.2108085Y30.

摘　　要：目的:通过将携带不同荧光蛋白基因的逆向跨多突触示踪病毒伪狂犬病病毒(PRV)分别注射入心肌与神门穴,观察示踪病毒在脑内核团的共标情况,探究神门-心脏与脑的神经联系.方法:选取C57BL/6小鼠6只,分别在小鼠的左心室壁和左上肢神门穴区注射携带绿色荧光蛋白基因的伪狂犬病病毒531(PRV531)和携带红色荧光蛋白基因的伪狂犬病病毒724(PRV724).于120 h后经4%多聚甲醛灌流取脑,做全脑切片.在荧光显微镜下观察示踪病毒标记下的神经元共标情况.结果:在小鼠大脑皮层、下丘脑、中脑、脑桥、延髓等各级中枢区域均发现了来自小鼠心室壁和神门穴区的共标信号.其中初级运动区、下丘脑室旁核、蓝斑下核及旁正中网状核中共标神经元数量较多.结论:神门、心脏与脑之间存在神经联系,这种联系可能与自主神经系统关系最为密切.Objective To investigate the neural connections between Shenmen(HT7)-heart and the brain by observing the tracing viruses co-labeled brain nuclear groups after injection of the pseudorabies viruses(PRV),the reverse transsynaptic virus tracer carrying different fluorescent protein genes,into the myocardium and Shenmen(HT7)point,respectively.Methods Pseudorabies virus 531(PRV531)carrying the green fluorescent protein gene and pseudorabies virus 724(PRV724)carrying the red fluorescent protein gene were injected into the left ventricular wall and Shenmen(HT7)point area of the left forelimb of six C57BL/6 mice,respectively.After 120 h,whole brain tissue was extracted under 4%paraformaldehyde perfusion to prepare brain sections.Neuronal co-labeling with the tracing viruses was observed under fluorescence microscopy.Results Co-labeled signals from the mouse ventricular wall and Shenmen(HT7)point region were found at all levels of the mouse central nervous areas,such as the cerebral cortex,hypothalamus,midbrain,pons,and medulla oblongata.The number of co-labeled neurons was higher in the primary motor area,the hypothalamic paraventricular nucleus,the subceruleus nucleus,and the paramedian reticular nucleus.Conclusion There is a neural connection between Shenmen(HT7),the heart,and the brain,which may be most closely related to the autonomic nervous system.

关 键 词：穴 神门 心脏 嗜神经病毒 伪狂犬病病毒 神经示踪技术 自主神经系统 小鼠 

分 类 号：R2-03[医药卫生—中医学]