Effects of nanopillars and surface coating on dynamic traction force  

作　　者：Yijun Cheng Stella W.Pang 

机构地区：[1]Department of Electrical Engineering,City University of Hong Kong,Kowloon,Hong Kong,China [2]Centre for Biosystems,Neuroscience,and Nanotechnology,City University of Hong Kong,Kowloon,Hong Kong,China

出　　处：《Microsystems & Nanoengineering》2023年第1期177-190,共14页微系统与纳米工程（英文）

基　　金：supported by the Center for Biosystems,Neuroscience,and Nanotechnology (CBNN) of the City University of Hong Kong (9360148,9380062);the University Grants Council of Hong Kong (GRF Projects:11212519,11207620,and 11207821).

摘　　要：The extracellular matrix serves as structural support for cells and provides biophysical and biochemical cues for cell migration.Topography,material,and surface energy can regulate cell migration behaviors.Here,the responses of MC3T3-E1 cells,including migration speed,morphology,and spreading on various platform surfaces,were investigated.Polydimethylsiloxane(PDMS)micropost sensing platforms with nanopillars,silicon oxide,and titanium oxide on top of the microposts were fabricated,and the dynamic cell traction force during migration was monitored.The relationships between various platform surfaces,migration behaviors,and cell traction forces were studied.Compared with the flat PDMS surface,cells on silicon oxide and titanium oxide surfaces showed reduced mobility and less elongation.On the other hand,cells on the nanopillar surface showed more elongation and a higher migration speed than cells on silicon oxide and titanium oxide surfaces.MC3T3-E1 cells on microposts with nanopillars exerted a larger traction force than those on flat PDMS microposts and had more filopodia and long protrusions.Understanding the relationships between platform surface condition,migration behavior,and cell traction force can potentially lead to better control of cell migration in biomaterials capable of promoting tissue repair and regeneration.

关 键 词：COATING TRACTION SURFACE 

分 类 号：TB383[一般工业技术—材料科学与工程]