A spiral microfluidic device for rapid sorting,trapping,and long-term live imaging of Caenorhabditis elegans embryos  

作　　者：Peng Pan Zhen Qin William Sun Yuxiao Zhou Shaojia Wang Pengfei Song Yong Wang Changhai Ru Xin Wang John Calarco Xinyu Liu 

机构地区：[1]Department of Mechanical and Industrial Engineering,University of Toronto,5 King’s College Road,Toronto,Ontario,M5S 3G8,Canada [2]Upper Canada College,200 Lonsdale Road,Toronto,Ontario,M4V 1W6,Canada [3]School of Advanced Technology,Xi’an Jiaotong-Liverpool University,111 Ren’ai Road,Suzhou,215000,China [4]School of Electronic and Information Engineering,Suzhou University of Science and Technology,Suzhou,215009,China [5]Department of Mechanical and Aerospace Engineering,Jilin University,Changchun,130012,China [6]Department of Cell&Systems Biology,University of Toronto,25 Harbord St,Toronto,Ontario,M5S 3G5,Canada [7]Institute of Biomedical Engineering,University of Toronto,164 College Street,Toronto,Ontario,M5S 3G9,Canada

出　　处：《Microsystems & Nanoengineering》2023年第1期203-213,共11页微系统与纳米工程（英文）

基　　金：supported by the Natural Sciences and Engineering Research Council of Canada (grant numbers:RGPIN-2017-06374,RGPAS-2017-507980,and RGPIN-2022-05039);the Canadian Institutes of Health Research (grant number:PJT-180365);the Canada Foundation for Innovation (grant number:JELF-38428);support from the National Natural Science Foundation of China (62273247);the Natural Science Foundation of the Jiangsu Higher Education Institutions of China (20KJA460008)to C.Ru is acknowledged;support from the Natural Science Foundation of the Jiangsu Higher Education Institutions of China (20KJB460024);the Young Scholar Program of Jiangsu Science and Technology (BK2020041995).

摘　　要：Caenorhabditis elegans embryos have been widely used to study cellular processes and developmental regulation at early stages.However,most existing microfluidic devices focus on the studies of larval or adult worms rather than embryos.To accurately study the real-time dynamics of embryonic development under different conditions,many technical barriers must be overcome;these can include single-embryo sorting and immobilization,precise control of the experimental environment,and long-term live imaging of embryos.This paper reports a spiral microfluidic device for effective sorting,trapping,and long-term live imaging of single C.elegans embryos under precisely controlled experimental conditions.The device successfully sorts embryos from a mixed population of C.elegans at different developmental stages via Dean vortices generated inside a spiral microchannel and traps the sorted embryos at single-cell resolution through hydrodynamic traps on the sidewall of the spiral channel for long-term imaging.Through the well-controlled microenvironment inside the microfluidic device,the response of the trapped C.elegans embryos to mechanical and chemical stimulation can be quantitatively measured.The experimental results show that a gentle hydrodynamic force would induce faster growth of embryos,and embryos developmentally arrested in the high-salinity solution could be rescued by the M9 buffer.The microfluidic device provides new avenues for easy,rapid,high-content screening of C.elegans embryos.

关 键 词：LARVAL SPIRAL TRAPPING 

分 类 号：Q95[生物学—动物学]