Gut-on-a-chip for exploring the transport mechanism of Hg(II)  

作　　者：Li Wang Junlei Han Weiguang Su Anqing Li Wenxian Zhang Huimin Li Huili Hu Wei Song Chonghai Xu Jun Chen 

机构地区：[1]School of Mechanical Engineering,Qilu University of Technology(Shandong Academy of Sciences),Jinan,250353,China [2]Shandong Institute of Mechanical Design and Research,Jinan,250353,China [3]The Key Laboratory of Experimental Teratology,Ministry of Education and Department of Genetics,School of Basic Medical Sciences,Shandong University,250012,Jinan,China [4]The Research Center of Stem Cell and Regenerative Medicine,School of Basic Medical Sciences,Cheeloo Medical College,Shandong University,250012,Jinan,China [5]Department of Oncology,Shandong Provincial Hospital Affiliated to Shandong University,Jinan,Shandong,250021,China

出　　处：《Microsystems & Nanoengineering》2023年第1期215-227,共13页微系统与纳米工程（英文）

基　　金：supported by the Taishan Scholars Program of Shandong Province (No.tsqn201812087);the National Natural Science Foundation of China (No.61901239);Qingchuang Science and Technology Plan Foundation for Colleges and Universities in Shandong Province (No.2019KJB009);the Young Ph.D.Cooperation Foundation of Qilu University of Technology (Shandong Academy of Sciences) (No.2019BSHZ002).

摘　　要：Animal models and static cultures of intestinal epithelial cells are commonly used platforms for exploring mercury ion(Hg(II))transport.However,they cannot reliably simulate the human intestinal microenvironment and monitor cellular physiology in situ;thus,the mechanism of Hg(II)transport in the human intestine is still unclear.Here,a gut-on-a-chip integrated with transepithelial electrical resistance(TEER)sensors and electrochemical sensors is proposed for dynamically simulating the formation of the physical intestinal barrier and monitoring the transport and absorption of Hg(II)in situ.The cellular microenvironment was recreated by applying fluid shear stress(0.02 dyne/cm^(2))and cyclic mechanical strain(1%,0.15 Hz).Hg(II)absorption and physical damage to cells were simultaneously monitored by electrochemical and TEER sensors when intestinal epithelial cells were exposed to different concentrations of Hg(II)mixed in culture medium.Hg(II)absorption increased by 23.59%when tensile strain increased from 1%to 5%,and the corresponding expression of Piezo1 and DMT1 on the cell surface was upregulated.

关 键 词：INTESTINE TRANSPORT mechanism 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置]