炔雌醇对绝经后骨质疏松症模型小鼠骨质量的影响  

Effects of ethinylestradiol on bone quality in postmenopausal osteoporosis mice

作　　者：王龙飞 高玉海 杨世超 李幸 孟盼盼 吴西 王中琪 魏娟娟 陈克明 WANG Long-fei;GAO Yu-hai;YANG Shi-chao;LI Xing;MENG Pan-pan;WU Xi;WANG Zhong-qi;WEI Juan-juan;CHEN Ke-ming(Basic Medical Laboratory,No.940 Hospital of Joint Logistics Support force of Chinese PLA,Lanzhou 730050,China;College of Life Science and Technology,Gansu Agricultural University,Lanzhou 730070,China;Gansu Key Laboratory of stem cells and genetic drug,Lanzhou 730050,China)

机构地区：[1]中国人民解放军联勤保障部队第九四〇医院基础医学实验室,甘肃兰州730050 [2]甘肃农业大学生命科学技术学院,甘肃兰州730070 [3]甘肃省干细胞与基因药物重点实验室,甘肃兰州730050

出　　处：《现代药物与临床》2023年第4期749-754,共6页Drugs & Clinic

基　　金：国家自然科学基金面上项目(81770879);甘肃省青年科技基金计划项目(20JR5RA589);联勤保障部队第九四〇医院实验室培育项目(2021yxky081)。

摘　　要：目的探究炔雌醇对绝经后骨质疏松症模型小鼠骨质量的影响。方法将30只SPF级C57雌性小鼠随机分为3组,每组10只,分别为假手术组、模型组和炔雌醇组,炔雌醇组每天ig炔雌醇100μg/kg,假手术组和模型组均ig给予等体积蒸馏水,给药12周后取材,分别观察脏器系数、主要脏器和骨组织病理学切片,分析Micro CT、生物力学和血清生化指标。结果与模型组相比,炔雌醇组小鼠体质量显著下降(P<0.05、0.01)。炔雌醇组的脏器系数除了子宫与模型组和假手术组有显著性差异,其余均没有显著性差异,主要脏器未发现明显的病变。骨组织脱钙切片显示,与模型组相比,炔雌醇组骨小梁结构致密,脂肪细胞较少。与模型组相比,炔雌醇组生物力学的最大载荷和弹性模量得到了明显的提高(P<0.05、0.01)。与模型组比较,炔雌醇组小鼠的骨小梁骨密度(Tb.BMD)、骨小梁数(Tb.N)、骨小梁厚度(Tb.Th)和骨体积分数(Tb.BV/TV)水平均显著增加,骨小梁分离度(Tb.Sp)数值显著减少(P<0.01)。与模型组比较,炔雌醇组血清骨钙素(OCN)和I型前胶原N端前肽(PINP)的平均水平显著升高;抗酒石酸酸性磷酸酶5b(TRACP-5b)和I型胶原交联C端肽(CTXI)水平显著降低(P<0.01)。结论炔雌醇能提高绝经后骨质疏松症模型小鼠骨质量同时对小鼠主要脏器没有损害。Objective To investigate the effects of ethinylestradiol on bone quality in postmenopausal osteoporosis mice.Methods Thirty SPF C57 female mice were randomly divided into 3 groups with 10 mice in each group:sham operation group,model group,and ethinylestradiol group.Ethinylestradiol group was given 100µg/kg ethinylestradiol intragastrically every day,sham operation group and model group were given equal volume of distilled water intragastrically.Samples were taken 12 weeks after administration,and organ coefficients,major organs and bone histopathological sections were observed,respectively,and Micro CT,biomechanical and serum biochemical indexes were analyzed.Results Compared with model group,the body weight of ethinylestradiol group was significantly decreased(P<0.05,0.01).The organ coefficients of ethinylestradiol group mice showed no significant differences except uterus,model group,and sham operation group,and no obvious lesions were found in the main organs.Compared with the model group,the bone trabecular structure in the ethinylestradiol group was dense and there were fewer fat cells.Compared with the model group,the maximum biomechanical load and elastic modulus of ethinylestradiol group were significantly increased(P<0.05,0.01).Compared with model group, the Tb.BMD, Tb.N, Tb.Th, and Tb.BV/TV levels of ethinylestradiol group were significantly increased, while the Tb.Sp value was significantly decreased (P < 0.01). Compared with model group, the mean serum OCN and PINP levels in ethinylestradiol group were significantly increased, TRACP-5b and CTX-I levels were significantly decreased (P < 0.01). Conclusion Ethinylestradiol can improve the bone quality of postmenopausal osteoporosis mice without damaging the major organs.

关 键 词：炔雌醇 脏器病理学 骨组织脱钙 骨密度 生物力学 骨代谢 

分 类 号：R977.1[医药卫生—药品]