健脾补肾解毒方通过JAK2/STAT3通路抑制肝癌上皮间质转化的研究  

Study on Jianpi Bushen Jiedu Decoction for inhibiting epithelial-mesenchymal transformation of hepatocellular carcinoma through JAK2/STAT3 pathway

作　　者：张平平 李会娣 刘宏杰[1] 朱晓骏[2] Zhang Pingping;Li Huidi;Liu Hongjie;Zhu Xiaojun(Department of Oncology,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Department of Hepatitis,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)

机构地区：[1]上海中医药大学附属曙光医院肿瘤科,上海201203 [2]上海中医药大学附属曙光医院肝病科,上海201203

出　　处：《国际中医中药杂志》2023年第5期581-585,共5页International Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81774256);上海中医药大学预算内课题(2019LK011)。

摘　　要：目的研究健脾补肾解毒方通过JAK2/STAT3通路对小鼠HCCLM3皮下移植瘤上皮间质转化的影响。方法构建小鼠HCCLM3皮下移植瘤模型。将造模成功的24只裸鼠按随机数字表法分为对照组、中药组、联合组,每组8只。中药组灌胃健脾补肾解毒方醇提物0.68 mg/ml,联合组灌胃索拉非尼混悬液3.5 mg/ml加健脾补肾解毒方醇提物0.68 mg/ml,1次/d,连续给药4周。观察各组小鼠HCCLM3皮下移植瘤体积、瘤重、小鼠体重;采用Western blot法检测HCCLM3皮下移植瘤组织中上皮间质转化相关蛋白钙黏蛋白E(E-cadherin)、钙黏蛋白N(N-cadherin)、波形蛋白(Vimentin)及JAK2/STAT3通路相关蛋白表达情况。结果与对照组比较,中药组、联合组小鼠HCCLM3皮下移植瘤平均瘤重降低(P<0.05),皮下移植瘤组织JAK2、STAT3、p-JAK2、p-STAT3、N-cadherin、Vimentin表达降低(P<0.05),E-cadherin表达升高(P<0.05)。结论健脾补肾解毒方对小鼠HCCLM3皮下移植瘤生长有抑制作用,其机制可能与抑制JAK2/STAT3通路相关蛋白活化,从而抑制肝癌上皮间质转化有关。Objective To study the effects of Jianpi Bushen Jiedu Decoction on the epithelial-mesenchymal transformation of nude mice with HCCLM3 subcutaneous transplanted tumor by regulating JAK2/STAT3 pathway.Methods HCCLM3 subcutaneous transplanted tumor model was established in mice.After the successful modeling,24 nude mice were divided into blank group,TCM group and combined group according to random number table method,with 8 mice in each group.Mice in the TCM group were given 0.68 mg/ml alcohol extract of Jianpi Bushen Jiedu Decoction for gavage,and the combined group were given sorafenib suspension plus alcohol extract of Jianpi Bushen Jiedu Decoction 3.5 mg/ml for gavage,once a day,for consecutive 4 weeks.The effects of Jianpi Bushen Jiedu Decoction on tumor volume,tumor weight of HCCLM3 subcutaneous transplanted tumor and mice body weight were observed;Western blot was used to detect the expressions of E-cadherin,N-cadherin,Vimentin and JAK2/STAT3 pathway-related proteins in subcutaneous transplanted tumor tissues of hepatocellular carcinoma of mice in each group.Results Compared with the control group,the average tumor weight of subcutaneous transplanted tumor decreased significantly in the TCM group and the combined group(P<0.05),and the expressions of JAK2,STAT3,p-JAK2,p-STAT3,N-cadherin,and Vimentin decreased significantly in subcutaneous transplanted tumor tissue(P<0.05),while E-cadherin increased(P<0.05).Conclusion Jianpi Bushen Jiedu Decoction can inhibit the growth of subcutaneous transplanted tumor of hepatocellular carcinoma in mice.The mechanism may be related to inhibiting the activation of JAK2/STAT3 pathway,thereby inhibiting the epithelial-mesenchymal transformation of hepatocellular carcinoma.

关 键 词：癌 肝细胞 钙粘着糖蛋白类 波形蛋白 健脾补肾解毒方 上皮间质转化 

分 类 号：R285.5[医药卫生—中药学]