高效液相色谱法同时测定藏药印度獐牙菜中9个成分  

Determination of 9 components Simultaneously in Swertia chirayita by HPLC method

作　　者：苏媛 杨增亮 刘安平 刘学良 刘海青 王开祥 石春兰 李维业 徐文胜 邹存生 Su Yuan;Yang Zengliang;Liu Anping;Liu Xueliang;Liu Haiqing;Wang Kaixiang;Shi Chunlan;Li Weiye;Xu Wensheng;Zou Cunsheng(Xining Center for Food and Drug Inspection&Testing,Xining 810007,China;The Drug Evaluation and Verification Center of Qinghai Province,Xining 810007,China;Qinghai Sanpu Pharmaceutical Co.,Ltd.,Xining 810007,China)

机构地区：[1]西宁市食品药品检验检测中心,西宁810007 [2]青海省药品审评核查中心,西宁810007 [3]青海三普药业有限公司,西宁810007

出　　处：《国际中医中药杂志》2023年第5期594-599,共6页International Journal of Traditional Chinese Medicine

基　　金：青海省基础研究项目(2020-ZJ747)。

摘　　要：目的建立HPLC法同时测定印度獐牙菜中9个成分含量的方法。方法用Waters SunFire C18柱(4.6 mm×250 mm,5μm);以甲醇-0.05%磷酸溶液为流动相进行梯度洗脱;柱温30℃,流速1.0 ml/min,检测波长为254 nm。结果獐牙菜苦苷、龙胆苦苷、当药苷、芒果苷、异荭草素、苦龙胆酯苷、当药醇苷、8-羟基-1,3,5三甲氧基酮、雏菊叶龙胆酮在进样质量范围内呈良好的线性关系,加样回收率分别为95.38%、92.41%、95.14%、91.87%、92.24%、92.51%、95.08%、91.72%、95.74%。结论该方法简便、高效、灵敏、准确、经济实用,具有良好的重复性和稳定性,为印度獐牙菜的质量控制、综合利用提供了有效参考依据。Objective To establish a HPLC method for determinating 9 components simultaneously in Swertia chirayita.Methods By useing water Sunfire C18 column(4.6 mm×250 mm,5μm);Gradient elution was carried out with methanol-0.05%phosphoric acid solution as mobile phase.Setting the column temperature at 30℃,the flow rate at 1.0 ml/min,and the detection wavelength at 254 nm.Results 9 components showed good linear relationship within the injection quality range.The recovery rates of wertiamarin,Gentiopicroside,Angelica glycosides,Mangiferin,Isolysine,Gentianoside,Diol glycoside,8-hydroxy-1,3,5 trimethoxyketone,and Daisy leaf gentinone were 95.38%,92.41%,95.14%,91.87%,92.24%,92.51%,95.08%,91.72%,95.74%(n=6).Conclusion The method is simple,efficient,sensitive,accurate,economical and practical,with repeatability and stability.It could provide reference for the quality control and comprehensive utilization of Swertia chirayita.

关 键 词：藏药 含量测定(中药) 印度獐牙菜 高效液相色谱法 质量控制 

分 类 号：R29[医药卫生—民族医学] O657.72[医药卫生—中医学]