构建大肠埃希菌底盘细胞合成N-糖基化蛋白的研究进展  

Advances in the construction of Escherichia coli chassis cells for the synthesis of N-glycosylated proteins

作　　者：包紫鑫 李威 胡学军[1] 丁宁[1] BAO Zixin;LI Wei;HU Xuejun;DING Ning(Dalian Key Laboratory of Glycan Recombination and Recombinant Protein Modification,Dalian University,Dalian 116622,China)

机构地区：[1]大连大学大连市糖重组及重组蛋白修饰重点实验室,大连116622

出　　处：《生命的化学》2023年第3期367-374,共8页Chemistry of Life

基　　金：国家自然科学基金项目(32070936,32270985);大连市科技创新基金项目(2022JJ13SN070)。

摘　　要：N-糖基化是自然界中主要的翻译后修饰之一,对蛋白质结构和功能的影响十分重要。随着糖工程领域的快速发展,在大肠埃希菌(Escherichia coli)中完成治疗性蛋白的N-糖基化修饰变得更加普遍。利用基因编辑技术对大肠埃希菌基因组进行编辑,使大肠埃希菌获得新的性状和生产能力,可以提高目标糖蛋白的产量。本文综述了通过基因编辑技术改造大肠埃希菌基因组来构建大肠埃希菌底盘细胞,及在此基础上优化N-糖基化效率以提高N-糖基化蛋白产量的研究进展,为构建具有N-糖基化修饰功能的工程菌株提供依据,为更好地进行糖蛋白生产,及进一步高效开发“糖蛋白工厂”提供策略。N-glycosylation is one of the major post-translational modifications in nature,and its effects on protein structure and function are important.With the rapid development in the field of glycoengineering,it has become more common to accomplish N-glycosylation modifications of therapeutic proteins in Escherichia coli(E.coli).Through the use of gene editing technology,E.coli genome can be modified to gain new features and productivity,which can boost the production of target glycoproteins.Using gene editing technology to modify the E.coli genome and create E.coli chassis cells,as well as improving N-glycosylation efficiency to increase the synthesis of N-glycosylated proteins,are discussed in this study.In addition to providing a foundation for the creation of engineered strains with N-glycosylation modification,this work offers techniques for increasing the efficiency of"glycoprotein factories"and improving glycoprotein output.

关 键 词：大肠埃希菌 基因编辑 基因组 N-糖基化修饰 糖基化效率 

分 类 号：Q78[生物学—分子生物学]