1株海洋来源真菌Cladosporium halotolerans次级代谢产物的研究  

Study on secondary metabolites of a marine fungus Cladosporium halotolerans

作　　者：刘国萍 张文婧 唐旭利[2] 李平林[1] 李国强[1] LIU Guo-ping;ZHANG Wen-jing;TANG Xu-li;LI Ping-lin;LI Guo-qiang(Key Laboratory of Marine Drugs,Ministry of Education,School of Medicine and Pharmacy,Ocean University of China,Qingdao 266003,China;College of Chemistry and Chemical Engineering,Ocean University of China,Qingdao 266100,China)

机构地区：[1]中国海洋大学海洋药物教育部重点实验室,医药学院,山东青岛266003 [2]中国海洋大学化学化工学院,山东青岛266100

出　　处：《中国海洋药物》2023年第2期59-63,共5页Chinese Journal of Marine Drugs

基　　金：国家自然科学基金面上项目(41876161)资助。

摘　　要：目的对1株来源于中国西沙群岛的软珊瑚Sarcophyton trocheliophorum共附生真菌Cladosporium halotolerans(18XS79ZP-2)进行次级代谢产物的化学成分研究。方法利用薄层色谱、硅胶柱层析、高效液相色谱(HPLC)和中压制备液相色谱(MPLC)等分离手段对真菌Cladosporium halotolerans的次级代谢产物进行分离、纯化;运用核磁共振波谱、质谱等方法,并通过与报道的数据进行对比,对化合物的化学结构进行鉴定。结果从真菌Cladosporium halotolerans的次级代谢产物中分离鉴定出10个化合物:1-Acetyl-β-carboline(1)、(-)-3a-hydroxyfuroindoline(2)、3-Hydroxyacetylindole(3)、(R)-3-Hydroxy-3-(2-hydroxyethyl)indolin-2-one(4)、(R)-3-hydroxy-3-(2’-oxopro-pyl)indolin-2-one(5)、Nb-acetyltryptamine(6)、cyclo-(L-Pro-L-Leu)(7)、cyclo-(L-Pro-L-Phe)(8)、cyclo-(L-Val-L-Tyr)(9)和cyclo-(L-Val-L-Leu)(10)。结论从真菌Cladosporiumhalotolerans的次级代谢产物中分离得到10个已知化合物,化学结构类型分别属于β-Carboline类生物碱、吲哚类生物碱和二酮哌嗪类生物碱。Objective To study the chemical constituents of secondary metabolites from a Sarcophyton trocheliophorum epiphyte Cladosporium halotolerans(18XS79ZP-2)from Paracel Islands,China.Methods The secondary metabolites of Cladosporium halotolerans were isolated and purified by TLC,silica gel column chromatography,HPLC and MPLC;The chemical structures of the compounds were identified by modern spectroscopy methods such as NMR and MS,and by comparison with the data of literature.Results Ten compounds were isolated and identified from the secondary metabolites of the fungus Cladosporium halotolerans which were identified as 1-Acetyl-β-carboline(1),(K)-3a-hydroxyfuroindoline(2),3-Hydroxyacetylindole(3),(R)-3-Hydroxy-3-(2-hydroxyethyl)indolin-2-one(4),(R)-3-hydroxy-3-(2"-oxopro-pyl)indolin-2-one(5),Nb-acetyltryptamine(6),cyclo-(L-Pro-L-Leu)(7),cyclo(L-Pro-L-Phe(8),cyclo-(L-Val-L-Tyr)(9),and cyclo-(L-Val-L-Leu)(10).Conclusion Ten known compounds were isolated from the secondary metabolites of the fungus Cladosporium halotolerans.Their chemical structures belong toβ-carboline alkaloid,indole alkaloids and diketopiperazine alkaloids respectively.Compounds 1~10 were obtained from the fungus Cladosporium halotolerans for the first time.

关 键 词：Cladosporium halotolerans 次级代谢产物 结构鉴定 

分 类 号：R931[医药卫生—生药学]