Light-Harvesting Artificial Cells for the Generation of ATP and NADPH  

作　　者：Ziwei Su Xiangxiang Zhang Weichen Wang Mingdong Dong Xiaojun Han 

机构地区：[1]State Key Laboratory of Urban Water Resource and Environment,School of Chemistry and Chemical Engineering,Harbin Institute of Technology,92 West Da-Zhi Street,Harbin,Heilongjiang 150001,China [2]Interdisciplinary Nanoscience Center(iNANO),Aarhus University,Aarhus DK-8000,Denmark

出　　处：《Chinese Journal of Chemistry》2023年第1期57-63,共7页中国化学（英文版）

基　　金：supported by the National Natural Science Foundation of China(Grant Nos.21929401,,22174031,22111540252,21773050);the Fundamental Research Funds forthe Central Universities(HIT.OCEF.2021026);the Heilongjiang Touyan Team(HITTY-20190034);the Natural Science Foundation of Heilongjiang Province(ZD2022B001).

摘　　要：The bottom-up construction of self-powered artificial cells is significant to understand the energy supply and metabolism of nature cells.Here,we demonstrate an efficient manner to build thylakoid-containing artificial cells,which continuously convert light energy into chemical energy to supply adenosine 5'-triphosphate(ATP)under light illumination.The production of ATP supplies energy to promote the biological enzyme cascade reactions,where glucose is transformed into glucose 6-phosphate(G6P)under the catalysis of hexokinase(HK).G6P was further converted to gluconolactone 6-phosphate(PG)in the presence of 6-phosphate dehydrogenase(G6PDH),meanwhile NADP^(+) was converted to nicotinamide adenine dinucleotide phosphate(NADPH).The self-powered artificial cells were demonstrated to generate ATP and NADPH successively,which provided a way for building more complicated artificial cells.

关 键 词：Artificial cells THYLAKOIDS METABOLISM ENZYMES Vesicles Light harvesting ATPNADPH GUVs 

分 类 号：Q813[生物学—生物工程]