苹果MdTOPP13/28在腋芽萌发中的表达分析  

Expression Analysis of MdTOPP13/28 During Axillary Bud Outgrowth in Malus

作　　者：刘柚藓 李国防 檀鸣 杨志昌 周世伟 霍文静 张鹤[1] 孙建设[1] 邵建柱[1] LIU Youxian;LI Guofang;TAN Ming;YANG Zhichang;ZHOU Shiwei;HUO Wenjing;ZHANG He;SUN Jianshe;SHAO Jianzhu(College of Horticulture,Hebei Agricultural University,Baoding,Hebei 071001,China)

机构地区：[1]河北农业大学园艺学院,河北保定071001

出　　处：《园艺学报》2023年第4期697-712,共16页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(31901981);国家现代农业产业技术体系建设专项资金项目(CARS-27)。

摘　　要：利用苹果全基因组数据,对苹果TOPP基因家族进行鉴定和生物学分析。结果表明:苹果TOPP基因家族共44个成员,分布于15条染色体上;系统进化树分析表明,苹果、桃和拟南芥TOPP高度同源;苹果TOPP基因结构中含1~2个外显子和0~20个内含子,以及10个保守基序;启动子顺式作用元件分析表明,苹果TOPP基因家族成员不仅受到光、热等环境影响,还受多种激素综合调控;基因芯片表达谱分析结果表明该基因家族在不同组织中均有表达。分析外源细胞分裂素(6-BA和TDZ)诱导苹果腋芽萌发的转录组数据,锁定了与其相关的候选基因MdTOPP13和MdTOPP28;克隆其序列并进行比对,两者表现出高同源性。以苹果砧木品种‘SH40’腋芽为材料,实时定量PCR分析表明,外源6-BA和TDZ处理后上调了MdTOPP13和MdTOPP28在腋芽中的表达量。综上可知,MdTOPP13和MdTOPP28在介导细胞分裂素调控腋芽萌发过程中可能发挥着重要作用。In this study,apple whole-genome data were used to identify and conduct a biological analysis of the MdTOPP gene family.A total of 44 TOPP genes were systematically identified from apple genome,and they distributed on 15 chromosomes.The phylogenetic tree analysis showed that MdTOPP,PpTOPP and AtTOPP were highly homologous;the gene structure and conserved domain analysis showed that 1-2 exons,0-20 introns and 10 conserved motifs were in MdTOPP genes.The promoter cis-acting element analysis showed that the MdTOPP genes were not only affected by the external environment such as light and heat,but also comprehensively regulated by multiple hormones.All the MdTOPP genes have distinctive expression patterns in different apple tissues.MdTOPP13 and MdTOPP28 were identified from the transcriptome data of exogenous cytokinin(6-BA and TDZ)induced apple axillary bud outgrowth,and cloning sequence alignment showed high homology.Using quantitative real-time PCR,the expressions of MdTOPP13 and MdTOPP28 were upregulated after exogenous 6-BA or TDZ applied on the axillary buds of apple rootstock‘SH40’.In summary,MdTOPP13 and MdTOPP28 may play an important role in mediating cytokinin regulation of axillary bud outgrowth.

关 键 词：苹果 TOPP 腋芽 萌发 细胞分裂素 

分 类 号：S666.1[农业科学—果树学]