糙皮侧耳pal2调控低温光照环境刺激下菌丝生长的分子机制  

The Molecular Mechanism of Phenylalanine Ammonia-Lyase Gene pal2 Regulating Pleurotus ostreatus Mycelial Growth Under Stimulation of Low-temperature and Light

作　　者：降玉清 何琪 侯潞丹 邬向丽[1] 黄晨阳[1] 赵梦然[1] JIANG Yuqing;HE Qi;HOU Ludan;WU Xiangli;HUANG Chenyang;ZHAO Mengran(Key Laboratory of Microbial Resources Collection and Preservation,Ministry of Agriculture and Rural Affairs,Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences,Beijing 100081,China;Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi,Jilin Agricultural University,Jilin,Changchun 130118,China;Key Laboratory of Shanxi Province for Loess Plateau Edible Fungi,Collaborative Innovation Center of Quality and Efficiency of Loess Plateau Edible Fungi in Shanxi,College of Food Science and Engineering,Shanxi Agricultural University,Jinzhong,Shanxi 030801,China)

机构地区：[1]中国农业科学院农业资源与农业区划研究所,农业农村部农业微生物资源收集与保藏重点实验室,北京100081 [2]吉林农业大学食药用菌教育部工程研究中心,吉林长春130118 [3]山西农业大学食品科学与工程学院,山西省食用菌工程技术研究中心,黄土高原食用菌山西省重点实验室,山西晋中030801

出　　处：《园艺学报》2023年第4期831-841,共11页Acta Horticulturae Sinica

基　　金：中国农业科学院所长基金项目(1610132022030);国家现代农业产业技术体系建设专项资金项目(CARS-20)。

摘　　要：以糙皮侧耳野生型菌株和pal2基因干扰菌株为材料,通过对比菌丝在28℃始终黑暗培养和28℃黑暗培养3 d后进行15℃12 h光照/12 h黑暗培养6 d的环境刺激这两种条件下苯丙氨酸解氨酶(phenylalanine ammonia-lyase,PAL)基因pal2转录水平、酶活性、底物L–苯丙氨酸和产物反式肉桂酸含量的变化,以及分析外源添加不同浓度底物和产物对菌丝表型的影响,初步解释了环境刺激下菌丝生长速度减慢的原因,发现苯丙氨酸解氨酶及其编码基因pal2均能响应环境刺激,但酶活性、产物和底物含量与基因表达量相比存在明显地响应滞后性;阐明环境刺激通过诱导pal2转录水平下调,总酶活性升高,使菌丝胞内L–苯丙氨酸减少,反式肉桂酸含量增加,从而负调控糙皮侧耳菌丝生长的分子通路。Wild-type strain and pal2 gene interference strains of Pleurotus ostreatus were used as materials in this study.All the strains were incubated at 28℃in the darkness for three days and then were treated with environmental stimulation(15℃,12 h light/12 h darkness)for six days.Strains incubated at 28℃in the darkness without the former environmental stimulation were taken as control.The pal2 gene transcription level,enzyme activity,contents of enzyme substrate and product of all the strains treated with different environmental conditions were tested and compared.The effects of various concentrations of exogenous substrates and products on the mycelial phenotype were examined.The results preliminarily explained the reasons for the slowdown of mycelial growth under environmental stimulation.The results revealed that both phenylalanine ammonia-lyase and the gene pal2 responded to environmental stimulation,but there was an obivous response lag in enzyme activity,product and substrate content compared with the expression of pal2.It was further clarified that the environmental stimulation induced down-regulation of both pal2 gene transcription level,increased enzyme activity,which caused the reduction of L-phe content and the increase of t-CA content in the mycelia,and thus negatively regulated the mycelial growth of P.ostreatus.

关 键 词：糙皮侧耳 低温光照 苯丙烷类代谢途径 

分 类 号：S646.141[农业科学—蔬菜学]