不同杀菌剂对混合菌生物膜杀菌缓蚀剥离效果的影响  

Effect of different biocides on bactericidal inhibition and stripping of mixed bacteria biofilm

作　　者：胥聪敏[1] 陈月清 朱文胜 高豪然 王文渊 杨兴 XU Congmin;CHEN Yueqing;ZHU Wensheng;GAO Haoran;WANG Wenyuan;YANG Xing(School of Materials Science and Engineering,Xi′an Shiyou University,Xi′an 710065,Shaanxi,China;CNOOC Changzhou Paint and Coating Industry Research Institute Co.,Ltd.,Changzhou 213000,Jiangsu,China)

机构地区：[1]西安石油大学材料科学与工程学院,陕西西安710065 [2]中海油常州涂料化工研究院有限公司,江苏常州213000

出　　处：《钢铁研究学报》2023年第2期190-200,共11页Journal of Iron and Steel Research

基　　金：国家自然科学基金资助项目(51974245,21808182);陕西省重点研发计划资助项目(2020GY-234);西安石油大学材料科学与工程学院西安市高性能油气田材料重点实验室,西安石油大学“材料科学与工程”省级优势学科资助项目(YS37020203);西安石油大学研究生创新与实践能力培养项目(YCS20112021,YCS20112020)。

摘　　要：采用MPN计数法、腐蚀失重试验、电化学测试、表面分析等方法,研究了不同杀菌剂对碳钢在含SRB+IOB混合菌生物膜中的杀菌缓蚀剥离效果及腐蚀行为规律。结果表明:TTPC(三丁基正十四烷基氯化膦)+D-tyrosine对混合菌生物膜的杀菌缓蚀剥离效果最佳,对混合菌中SRB、IOB的杀菌率分别可达98.5%、85.6%,缓蚀率可达64.24%,其杀菌缓蚀效果优于加入单一杀菌剂1227、THPS(四羟甲基硫酸磷)、TTPC和复配杀菌剂。表面分析结果表明:加入TTPC+D-tyrosine后,碳钢表面的硫化物含量与腐蚀坑数量均显著降低,腐蚀速率明显减缓,生物膜快速分解,说明TTPC+D-tyrosine对混合菌的杀菌缓蚀剥离效果最佳;加入单一1227,生物膜会产生室阻效应,从而降低杀菌效果;加入单一TTPC、THPS后,带正电荷TTPC被带负电荷的细菌吸附到细菌内部,导致细菌变性失活,且TTPC可溶解SRB代谢产生的FeS,而THPS的亲油基团能溶解并损伤细胞表面的脂肪壁,同时抑制微生物生成的硫化物与磷化物,致使腐蚀速率下降,但灭菌效果远不及TTPC+D-tyrosine的。The corrosion behavior of carbon steel in the mixed bacteria biofilm containing SRB(sulfate reducing bacteria) and IOB(iron oxide bacteria)were studied by means of MPN counting method, corrosion weight loss experiment, electrochemical measurement and surface analysis methods. The results show that bacteriacide of TTPC+D-tyrosine has the best bactericidal and corrosion inhibition stripping effect on mixed bacteria biofilms, and the bactericidal rate of SRB and IOB in mixed bacteria can reach 98.5% and 85.6%, respectively, and the corrosion inhibition rate can reach 64.24%. Its bactericidal and corrosion inhibition effects are better than that of single bactericides such as 1227, THPS, TTPC and compound bacteriacide. The surface analysis results show that, the sulfide content and the number of corrosion pits on the surface of carbon steel were decreased significantly after adding TTPC+D-tyrosine, the corrosion rate was slowed down significantly, and the biofilm was decomposed rapidly, indicating that TTPC+D-tyrosine had the best bactericidal and corrosion inhibition stripping effect on the mixed bacteria, so TTPC+D-tyrosine is the best bactericide formula;the biofilm will produced a blocking effect, thus reduced the bactericidal effect after adding single 1227;after adding a single TTPC or THPS, the positively charged TTPC is adsorbed into the bacteria by the negatively charged bacteria, which lead to bacterial denaturation and inactivation, and TTPC can dissolve the FeS produced by SRB metabolism, while the lipophilic group of TTPC can dissolve and damage the fatty wall on the cell surface, at the same time, the sulphide and phosphide generated by microorganisms are inhibited, these factors reduced the corrosion rate of carbon steel, but the sterilization effect is much less than that of TTPC+D-tyrosine.

关 键 词：微生物腐蚀 杀菌剂 生物膜剥离 腐蚀机制 缓蚀率 

分 类 号：TG172.7[金属学及工艺—金属表面处理]