Systematic identification of CRISPR off-target effects by CROss-seq  

作　　者：Yan Li Shengyao Zhi Tong Wu Hong-Xuan Chen Rui Kang Dong-Zhao Mai Zhou Songyang Chuan He Puping Liang Guan-Zheng Luo 

机构地区：[1]IMOE Key Laboratory of Gene Function and Regulation,Guangdong Province Key Laboratory of Pharmaceutical Functional Genes,State Key Laboratory of Biocontrol,School of Life Sciences,Sun Yat-sen University,Guangzhou 510275,China [2]Department of Chemistry,University of Chicago,Chicago,IL 60637,USA [3]Institute for Biophysical Dynamics,Department of Biochemistry and Molecular Biology,Howard Hughes Medical Institute,University of Chicago,Chicago,IL 60637 USA

出　　处：《Protein & Cell》2023年第4期299-303,共5页蛋白质与细胞（英文版）

基　　金：supported by the Ministry of Science and Technology of China to G.Z.L.(National Science and Technology Major Project,grant nos.2018YFA0109100,2019YFA0802203);National Natural Science Foundation of China to G.z.L.(Grant Nos.31922015,31870808,91753129);Natural Science Foundation of Guangdong Province to G.Z.L.(Grant No.2018B030306044);Guangdong Special Support Program to P.L.(2019BT02Y276).

摘　　要：DearEditor,The CRISPR-mediated genome editing tools,including nucleases,base editors(ABE/CBE),transposases/recombinases,and prime editor(PE),have been extensively applied in basic and clinical researches,although the off-target effect remains a major concern(Anzalone et al.,2020).Recently,various methods have been developed to assess the specificity and accuracy of different tools(Zhang et al.,2021),yet each method is designed for limited editing systems,and none of them can simultaneously detect off-target sites in vivo and in vitro.A versatile method for profiling genome-wide off-target effects of various tools remains lacking.

关 键 词：CRISPR VERSATILE EDITOR 

分 类 号：Q78[生物学—分子生物学]