傣药“雅解”:宾蒿、傣百解及竹叶兰的生物活性及其解毒机制  

Biological activities and detoxification mechanisms of Clerodendrum chinense var.simplex,Marsdenia tenacissima and Arundina graminifolia:The Dai antidotes

作　　者：张雪飞 赵俊可 郝素旗 丁青 于世慧 伊木朗 王军[2] 胡海燕[2] ZHANG Xuefei;ZHAO Junke;HAO Suqi;DING Qing;YU Shihui;MOHAMMAD Imran shair;WANG Jun;HU Haiyan(School of Traditional Dai-Thai Medicine,West Yunnan University of Applied Sciences,Jinghong 666100,China;School of Pharmaceutical Sciences,Sun Yat-sen University,Guangzhou 510006,China;Yunnan Dai Medicine Co.,Ltd.,Ruili 678699,China)

机构地区：[1]滇西应用技术大学傣医药学院,云南景洪666100 [2]中山大学药学院,广东广州510006 [3]云南傣药有限公司,云南瑞丽678699

出　　处：《中山大学学报（自然科学版）（中英文）》2023年第3期89-99,共11页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：Yunnan Provincial Department of Education Science Research Fund Project(2018JS709);National Natural Science Foundation of China(81973264);Guangdong Basic and Applied Basic Research Foundation(2019A1515011954,2020A1515010593)。

摘　　要：傣药“雅解”是傣族传统医学中最具特色的药物和治疗方法。其中,宾蒿(Clerodendrum chinense var.simplex)、傣百解(Marsdenia tenacissima)和竹叶兰(Arundina graminifolia)作为3种常见的“雅解”被广泛使用。本研究使用石油醚、乙酸乙酯、正丁醇或水依次萃取3种“雅解”的乙醇提取物获得各极性部位,并制备3种“雅解”的水煎剂。经测定3种“雅解”的总黄酮质量分数为22.41~586.39 mg/g,高于总多酚质量分数(2.76~28.66 mg/g)。3种“雅解”的提取物均可清除DPPH、·OH和ABTS自由基,其中对ABTS自由基的清除率高于其他自由基(IC50>380μg/mL)。它们对大肠杆菌、铜绿假单胞菌和金黄色葡萄球菌的抑制作用较弱。此外,3种“雅解”具有一定的抗炎作用,在60μg/mL的质量浓度下即可显著下调脂多糖刺激巨噬细胞产生的NO、肿瘤坏死因子-α、白细胞介素-1β和白细胞介素-6。综上所述,本研究通过现代科学手段初步揭示了3种“雅解”的解毒作用机理,为“雅解”的进一步优化与研究奠定了基础。Dai antidotes are the most distinctive medicine and treatment in traditional Dai medicine.Bin Hao(Clerodendrum chinense var.simplex),Dai Bai Jie(Marsdenia tenacissima)and Zhu Ye Lan(Arundina graminifolia)are three Dai antidotes widely used for their"detoxifying effects",and their use is rooted in a theoretical system significantly different and much less understood than Western or traditional Chinese medicines.Here,we successively extracted the three Dai antidotes using petroleum ether,ethyl acetate,n-butanol,or water,and then prepared their decoctions.The content of total flavonoids in three Dai antidotes ranged from 22.41 to 586.39 mg/g,which is higher than the content of total polyphenols(2.76 to 28.66 mg/g).The various extracts were found to scavenge radicals of DPPH,·OH and ABTS.They scavenged ABTS radicals much more efficiently than other radicals(IC50>380μg/mL).They weakly inhibited the growth of E.coli,P.aeruginosa and S.aureus.Notably,even at low concentration 60μg/mL,the extracts can significantly down-regulate the production of NO,TNF-α,IL-1β,and IL-6 by macrophages stimulated with LPS.In conclusion,our results provide the first mechanistic insights into the detoxifying effects of three Dai antidotes,providing a foundation for their optimization and for future research to strengthen Dai medicine through modern scientific practices.

关 键 词：傣药“雅解” 宾蒿(Clerodendrum chinense var.simplex) 傣百解(Marsdenia tenacissima) 竹叶兰(Arundina graminifolia) 解毒作用 

分 类 号：R29[医药卫生—民族医学]