球孢白僵菌与四唑虫酰胺联合应用对草地贪夜蛾的毒力研究  

Study on the Toxicity of Beauveria bassiana Combined with Tetraniliprole to Spodoptera frugiperda

作　　者：蒋文杰 李伟 郑军 苏伶俐 李瑞鹏 张紫慧 雷云飞 任家琼 张开军[1] 王梓英[1] 刘怀[1] JIANG Wenjie;LI Wei;ZHENG Jun;SU Lingli;LI Ruipeng;ZHANG Zihui;LEI Yunfei;REN Jiaqiong;ZHANG Kaijun;WANG Ziying;LIU Huai(Key Laboratory of Entomology and Pest Control Engineering,College of Plant Protection,Southwest University,Chongqing 400715,China;Agricultural Technology Extension Station of Fuling District,Chongqing 408000,China;Agricultural Technology Extension Center of Jiangjin District,Chongqing 402260,China;Plant Protection and Inspection Station of Dianjiang County,Chongqing 408300,China)

机构地区：[1]西南大学植物保护学院/昆虫学及害虫控制工程重庆市市级重点实验室,重庆400715 [2]重庆市涪陵区农业技术推广站,重庆408000 [3]重庆市江津区农业技术推广中心,重庆402260 [4]重庆市垫江县植保植检站,重庆408300

出　　处：《植物医学》2023年第2期59-68,共10页Plant Health and Medicine

基　　金：鲁渝科技协作计划项目(cstc2020jscx-lyggX0006);重庆市现代山地特色高效农业蔬菜产业技术体系项目(2020[4-6]).

摘　　要：为了明确四唑虫酰胺与球孢白僵菌联合应用对草地贪夜蛾的防治效果,本研究选择了对草地贪夜蛾高毒力的球孢白僵菌Bb105株系和化学药剂四唑虫酰胺,开展了两者不同比例混配和不同使用顺序对草地贪夜蛾幼虫的毒力研究.球孢白僵菌与四唑虫酰胺不同配比对草地贪夜蛾毒力的研究结果表明,两者以9∶1和4∶1的比例混合使用时,共毒系数分别为738.50和421.19,表现出增效作用,且混用比例为9∶1时增效作用最明显.两者不同使用顺序对草地贪夜蛾的毒力测定结果表明,先使用球孢白僵菌72 h后再使用四唑虫酰胺对草地贪夜蛾的实际致死率比预测死亡率高,也表现出协同增效作用.研究结果为菌药联合防治草地贪夜蛾的实践应用奠定了良好的理论基础.In order to clarify the control effect of the combined application of tetraniliprole and Beauveria bassiana on the armyworm Spodoptera frugiperda,this study selected the B.bassiana Bb105 and the chemical Tetraniliprole,which are highly toxic to this pest,to carry out a toxicity study on the larvae of S.frugiperda by mixing them in different proportions and using them in different application order.The results of the study on the toxicity of different ratios of the B.bassiana and tetraniliprole to S.frugiperda showed that when the two were mixed at 9∶1 and 4∶1,the co-toxicity coefficients were 738.50 and 421.19,respectively,showing a synergistic effect,and the former had the most obvious synergistic effect.The toxicity test results of the two different order of uses on S.frugiperda showed that using B.bassiana for 72 hours before using tetraniliprole has a higher actual mortality rate than the predicted mortality rate,also showed a synergistic effect.The research results have laid a good theoretical foundation for the practical application of the combination of entomopathogenic fungi and chemical in the control of S.frugiperda.

关 键 词：球孢白僵菌 四唑虫酰胺 草地贪夜蛾 联合毒力 

分 类 号：S435.132[农业科学—农业昆虫与害虫防治]