当归多糖对糖尿病KK-Ay小鼠心肌细胞凋亡的影响  

Effects of Angelica sinensis polysaccharide on the apoptosis of cardiomyocytes in diabetic KK-Ay mice

作　　者：崔阳阳 杨丽霞[2] 米登海 魏瑞贤 CUI Yangyang;YANG Lixia;MI Denghai;WEI Ruixian(Clinical College of Traditional Chinese Medicine,Gansu University of Traditional Chinese Medicine,Lanzhou 730099,China;Central Laboratory of Gansu Provincial Institute of Traditional Chinese Medicine,Lanzhou 730050,China)

机构地区：[1]甘肃中医药大学中医临床学院,兰州730099 [2]甘肃省中医药研究院中心实验室,兰州730050

出　　处：《中国药房》2023年第10期1211-1215,共5页China Pharmacy

基　　金：甘肃省科技计划项目(No.20JR10RA432)。

摘　　要：目的探讨当归多糖对糖尿病KK-Ay小鼠心肌细胞凋亡的影响。方法将KK-Ay小鼠随机分为模型组、二甲双胍组(200mg/kg)和当归多糖高、中、低剂量组(400、200、100 mg/kg),C57BL/6J小鼠作为空白组,每组8只,各组小鼠每日灌胃相应药液或生理盐水1次,连续4周。末次给药后,检测小鼠的空腹血糖、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、胰岛素(INS)水平,心肌组织中B细胞淋巴瘤2(Bcl-2)、活化的胱天蛋白酶(cleaved-caspase-3)、凋亡信号调节激酶1(ASK1)、磷酸化氨基末端蛋白激酶(p-JNK)、磷酸化需肌醇酶1α(p-IRE1α)蛋白表达以及心肌细胞凋亡情况。结果与模型组比较,二甲双胍组和当归多糖中、高剂量组小鼠空腹血糖、TC、LDL-C含量和心肌细胞凋亡率以及p-JNK、p-IRE1α、ASK1、cleaved-caspase-3蛋白表达水平均显著降低,INS水平和Bcl-2蛋白表达水平均显著升高(P<0.05或P<0.01)。结论当归多糖可改善糖尿病KK-Ay小鼠的血糖和血脂水平,抑制心肌细胞凋亡,其机制可能与抑制IRE1/ASK1/JNK信号通路有关。OBJECTIVE To investigate the effects of Angelica sinensis polysaccharide on the apoptosis of cardiomyocytes in diabetic KK-Ay mice.METHODS KK-Ay mice were randomly divided into model group,metformin group(200 mg/kg)and A.sinensis polysaccharide high-dose,medium-dose and low-dose groups(400,200 and 100 mg/kg);C57BL/6J mice were included in blank group,with 8 mice in each group.Each group was given relevant medicine intragastrically or normal saline,once a day,for consecutive 4 weeks.After the final administration,the levels of fasting glucose,total cholesterol(TC),low-density lipoprotein cholesterol(LDL-C)and insulin(INS)were detected;the protein expressions of B-cell lymphoma 2(Bcl-2),cleavedcaspase-3,apoptosis signal-regulated kinase 1(ASK1),phosphorylated c-Jun N-terminal kinase(p-JNK),phosphorylated inositolrequiring enzyme 1α(p-IRE1α)in myocardium,and apoptosis in cardiomyocytes were also detected.RESULTS Compared with model group,the fasting glucose,TC and LDL-C content,apoptotic rate of cardiomyocyte,protein expressions of p-JNK and p-IRE1α,ASK1,cleaved-caspase-3 were significantly lower in the metformin group and A.sinensis polysaccharide medium-dose,high-dose groups;INS level and relative expression of Bcl-2 protein were significantly increased(P<0.05 or P<0.01).CONCLUSIONS A.sinensis polysaccharide can improve the levels of blood glucose and blood lipid and inhibit cardiomyocyte apoptosis in diabetic KK-Ay mice,and the mechanism may be related to the inhibition of IRE1/ASK1/JNK signaling pathway.

关 键 词：当归多糖 糖尿病心肌病 KK-AY小鼠 细胞凋亡 IRE1/ASK1/JNK信号通路 

分 类 号：R965[医药卫生—药理学]