半乳糖凝集素1在退变椎间盘中的差异表达  

Differentially expressed galectin-1 during intervertebral disc degeneration

作　　者：梁卫东[1] 张树文 蔡晓宇 荀传辉[1] 盛军[1] 曹锐[1] 郝宏刚 盛伟斌[1,3,4] Liang Weidong;Zhang Shuwen;Cai Xiaoyu;Xun Chuanhui;Sheng Jun;Cao Rui;Hao Honggang;Sheng Weibin(Department of Spine Surgery,the First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054,Xinjiang Uygur Autonomous Region,China;Department of Spine Surgery II,People’s Hospital of Xinjiang Uygur Autonomous Region,Urumqi 830000,Xinjiang Uygur Autonomous Region,China;Key Laboratory of the Ministry of Education for High Incidence Diseases in Xinjiang Region(Xinjiang Medical University),Urumqi 830054,Xinjiang Uygur Autonomous Region,China;Xinjiang Clinical Research Center of Orthopaedic Diseases,Urumqi 830054,Xinjiang Uygur Autonomous Region,China)

机构地区：[1]新疆医科大学第一附属医院脊柱外科,新疆维吾尔自治区乌鲁木齐市830054 [2]新疆维吾尔自治区人民医院骨科脊柱二病区,新疆维吾尔自治区乌鲁木齐市830000 [3]新疆地区高发疾病研究教育部重点实验室(新疆医科大学),新疆维吾尔自治区乌鲁木齐市830054 [4]新疆骨科疾病临床医学研究中心,新疆维吾尔自治区乌鲁木齐市830054

出　　处：《中国组织工程研究》2023年第35期5610-5615,共6页Chinese Journal of Tissue Engineering Research

基　　金：新疆维吾尔自治区自然科学基金青年基金(2017D01C326),项目负责人:梁卫东。

摘　　要：背景:半乳糖凝集素1调控诸多组织、器官的生理病理过程,其与椎间盘退变的关系尚不明确。目的:分析半乳糖凝集素1在不同退变程度椎间盘中的差异性表达,并探讨其对大鼠椎间盘退变的影响。方法:收集新疆医科大学第一附属医院15例患者的椎间盘标本,采用Pfirrmann分级后检测半乳糖凝集素1在不同退变程度椎间盘中的差异性表达。建立SD大鼠腰椎退变模型,随机分为3组,造模后隔日进行干预,对照组、模型组分别尾静脉注射生理盐水,半乳糖凝集素1干预组尾静脉注射半乳糖凝集素1重组蛋白,1次/d,各组均干预7 d;造模术后8周使用Bruker Pharmascan 7.0T核磁共振仪进行Pfirrmann分级后观察椎间盘的病理变化,并检测椎间盘中半乳糖凝集素1、Ⅱ型胶原及蛋白聚糖的表达。结果与结论:①qRT-PCR、免疫组织化学染色及Western Blot结果提示随着椎间盘退变的加重,半乳糖凝集素1表达逐渐减少,3组之间比较差异有显著性意义(P<0.05);②术后8周,大鼠腰椎MRI提示半乳糖凝集素1干预可降低改良Pfirrmann分级;苏木精-伊红染色提示半乳糖凝集素1干预减少椎间盘退变的病理改变;免疫组化及Western blot结果提示半乳糖凝集素1干预在增加椎间盘中半乳糖凝集素1表达的同时减少Ⅱ型胶原及蛋白聚糖的降解,3组之间相比差异有显著性意义(P<0.05);③结果提示随着椎间盘退变程度的加重半乳糖凝集素1表达逐渐减少;半乳糖凝集素1干预可以延缓大鼠椎间盘针刺退变模型的进展。BACKGROUND:Galectin-1 is involved in the physiological and pathological processes of many tissues and organs,but its relationship with intervertebral disc degeneration remains unclear.OBJECTIVE:To analyze the differential expression of galectin-1 in intervertebral discs and to investigate the effect of galectin-1 on intervertebral disc degeneration in rats.METHODS:Intervertebral disc specimens were collected from 15 patients in the First Affiliated Hospital of Xinjiang Medical University.Pfirrmann grading was used to definite the degree of degeneration,and the differential expression of galectin-1 in intervertebral discs with different degrees of degeneration was detected.A lumbar degeneration model was established in Sprague-Dawley rats.The model rats were randomly divided into control group,model group and galectin-1 group.Interventions were carried out on alternate days after modeling.Control and model groups were given normal saline injection into the tail vein,while galectin-1 recombinant protein was injected into the tail vein of the galectin-1 group once a day for 7 days.Eight weeks after modeling,pathological changes in the intervertebral disc were observed after Pfirrmann grading using Bruker Pharmascan 7.0T MRI and the expression of galectin-1,type II collagen and Aggrecan in the disc was examined.RESULTS AND CONCLUSION:qRT-PCR,immunohistochemical staining and western blot analysis indicated that galectin-1 expression decreased gradually with the aggravation of intervertebral disc degeneration,and there were significant differences among three groups(P<0.05).At 8 weeks after modeling,MRI scan of the rat lumbar vertebra results indicated that galectin-1 could reduce the modified Pfirrmann grade of intervertebral disc degeneration,and hematoxylineosin staining suggested that galectin-1 could reduce the pathological manifestations of intervertebral disc degeneration.Immunohistochemistry and western blot results suggested that intervention with galectin-1 could increase the expression of galectin-1 in the i

关 键 词：半乳糖凝集素1 椎间盘 退变 差异表达 Ⅱ型胶原 蛋白聚糖 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] R453