不同果型翅果油树的抗寒性  

Cold Resistance of Elaeagnus mollisof Different Fruit Types

作　　者：王金鑫[1] 王延松 明文 王森[1] 王纪明 李彦慧[1] Wang Jinxin;Wang Yansong;Ming Wen;Wang Sen;Wang Jiming;Li Yanhui(Hebei Agricultural University,Baoding 071000,P.R.China;Beijing Landscape Design Engineering Co.,Ltd.;Tianya agriculture and Forestry Technology Group Co.,Ltd.)

机构地区：[1]河北农业大学,保定071000 [2]北京市园林设计工程有限公司 [3]天亚农林科技集团有限公司

出　　处：《东北林业大学学报》2023年第6期25-32,共8页Journal of Northeast Forestry University

基　　金：河北省重点研发计划项目(21326802D);河北省林业与草原局科技项目(1906006)。

摘　　要：以4种果型(毛果型、大果型、长果型、小果型)翅果油树(Elaeagnus mollis Diels.)的1年生枝条为试验材料,进行低温(4、-10、-20、-30、-40℃)处理,测定其丙二醛质量摩尔浓度、相对电导率、保护酶活性、渗透调节物质质量分数及电阻抗参数等指标,并以相对电导率为依据,探究电阻抗参数与抗寒性的关系,利用主成分分析和隶属函数法综合评价4种果型翅果油树的抗寒性。结果表明:随着处理温度的降低,4种果型翅果油树枝条的丙二醛(MDA)质量摩尔浓度和相对电导率均呈升高趋势,过氧化物酶(POD)活性、脯氨酸质量分数均呈先上升后下降的趋势,胞外电阻率、胞内电阻率、弛豫时间整体均呈逐渐降低的趋势,毛果型和大果型的超氧化物歧化酶(SOD)活性、大果型和小果型的可溶性糖质量分数、毛果型的可溶性蛋白质量分数均呈“上升-下降-再上升”的趋势,长果型和小果型的超氧化物歧化酶(SOD)活性、大果型和长果型的过氧化氢酶(CAT)活性、毛果型和长果型的可溶性糖质量分数、小果型的可溶性蛋白质量分数、大果型和长果型的弛豫时间分布系数均呈先上升后下降的趋势,毛果型和小果型的过氧化氢酶(CAT)活性呈“上升-下降-上升-再下降”的趋势,大果型的可溶性蛋白质量分数呈“下降-上升-再下降”的趋势,长果型的可溶性蛋白质量分数、毛果型和小果型的弛豫时间分布系数均呈“下降-上升-下降-再上升”的趋势;毛果型、大果型、长果型和小果型的半致死温度(T_(L,50))分别为-34.60、-30.27、-24.57、-35.18℃;相关性分析表明,各电阻抗参数均可作为评价翅果油树抗寒性的指标。综合分析表明,4种果型翅果油树的抗寒性由强到弱依次为小果型、毛果型、大果型、长果型。With four fruit types(hairy,large,long and small)of one-year-old branches of Elaeagnus mollis Diels.,the branches were treated with low temperature(4,-10,-20,-30 and-40℃).Mass molality of malondialdehyde(MDA),relative conductivity,protective enzyme activity,mass fraction of osmotic regulating substances and electrical impedance parameters were determined.From the relative conductivity,the relationship between electrical impedance parameters and cold resistance was explored.The cold resistance of four kinds of fruit types of E.mollis was comprehensively evaluated by principal component analysis and membership function method.With the decrease of treatment temperature,the MDA mass molar concentration and relative electrical conductivity of four kinds of fruit typesof E.mollis branches were increased,the peroxidase(POD)activity and proline mass fraction were increased first and then decreased,and the extracellular resistivity,intracellular resistivity and relaxation time were decreased gradually.The superoxide dismutase(SOD)activity of hairy fruit type and large fruit type,soluble sugar mass fraction of large fruit type and small fruit type,and soluble protein mass fraction of hairy fruit type showed a trend of“rising-decreasing-rising”.The SOD activity of longfruit type and smallfruit type,CAT activity of largefruit type and longfruit type,soluble sugar mass fraction of hairy fruit type and longfruit type,soluble protein mass fraction of smallfruity type,and relaxation time distribution coefficients of large fruit type and long fruit type were firstly increased and then decreased.The CAT activity of hairy fruit type and small fruit type showed a trend of“ascending-descending-ascending-descending”.The soluble protein mass fraction of large fruit type showed a trend of“descending-ascending-descending”.The soluble protein mass fraction of long fruit type and the relaxation time distribution coefficients of hairy fruit type and small fruit type showed a trend of“descending-ascending-descending-ascending

关 键 词：翅果油树 抗寒性 电阻抗参数 生理指标 主成分分析 

分 类 号：S792.99[农业科学—林木遗传育种]