陕产附子泡胆工艺的改进和优化研究  

Research on the improvement and optimization of the brine soaking technology of Shaanxi-produced crude aconite

作　　者：李凡[1] 张景霞 王卫锋[1] 李莎莎 毛阿娟 李雅娟 陈曦 李芳[1] LI Fan;ZHANG Jingxia;WANG Weifeng;LI Shasha;MAO Ajuan;LI Yajuan;CHEN Xi;LI Fang(Shaanxi Provincial Traditional Chinese Medicine Research Institute,Xi’an 710003,China)

机构地区：[1]陕西省中医药研究院中药研究所,西安710003 [2]西安国际医学中心有限公司药剂室 [3]陕西中医药大学药学院

出　　处：《环球中医药》2023年第5期867-874,共8页Global Traditional Chinese Medicine

基　　金：陕西省科技计划项目(2020SF-325)。

摘　　要：目的探讨氯化钙代替胆巴进行陕产附子泡胆的可能性和科学性,并对泡胆工艺参数进行优选。方法以单/双酯型生物碱的含量为指标,进行胆巴溶液浸泡与氯化钙溶液浸泡陕产附子的泡胆工艺平行比较研究,然后以效/毒为评价指标,通过单因素试验结合Box-Behnken响应面法,研究浸泡时间(分别浸泡6、12、18、24、30天,)、氯化钙浓度(分别加入浓度为10%、15%、20%、25%、30%的氯化钙溶液)、料液比(分别按料液比为1∶2、1∶3、1∶4、1∶5、1∶6加入25%的氯化钙溶液)对泡胆结果的影响,优选最佳泡胆工艺,并进行验证。结果陕产附子分别经过较长时间的胆巴溶液、氯化钙溶液浸泡后生物碱总量均有所下降,且下降趋势一致。响应面最优工艺为:浸泡时间为19天、料液比为1∶4、氯化钙浓度为25%。验证试验中,平均效/毒为(0.069±0.012),在理论值0.071的误差范围内。结论采用有药典标准的氯化钙代替胆巴作为附子防腐、加工和炮制的辅料。运用星点设计—效应面法,优选的陕产附子氯化钙泡胆工艺操作简便、稳定可靠,可为陕产附子的进一步炮制研究奠定基础。Objective Discuss the possibility and scientificity of calcium chloride instead of brine to soak Shaanxi-produced crude aconite,and optimize the brine process parameters.Methods Taking the content of mono/diester alkaloids as the index,a parallel comparative study was carried out on the bile soaking process of the aconite produced in Shaanxi province by soaking in bile solution and calcium chloride solution.Then using efficacy/toxicity as the evaluation index,the effects of soaking time(soaking for 6,12,18,24,and 30 days),calcium chloride concentration(adding 10%,15%,20%,25%,30%of calcium chloride),and material-to-liquid ratio(add 25%calcium chloride solution according to the material-to-liquid ratio of 1∶2,1∶3,1∶4,1∶5,1∶6 respectively)on the soaking process were studied by the single factor test and Box-Behnken response surface methodology,and the soaking process was optimized and verified.Results The total amount of alkaloids of aconite produced in Shaanxi was decreased after being soaked in brine solution and calcium chloride solution for a long time,and the downward trend was consistent.The optimal soaking process was as follows:the soaking time was 19 days,the material-to-liquid ratio was 1∶4,and the calcium chloride concentration was 25%.In the verification test,the average effect/toxicity was(0.069±0.012),which was within the error range of the theoretical value of 0.071.Conclusion Calcium chloride with pharmacopoeia standards can be used instead of brine as auxiliary materials for anti-corrosion and processing of crude aconite.Using the star-point design-response surface method,the optimized aconite calcium chloride soaking process is simple,stable and reliable,which can lay the foundation for the further research on aconite.

关 键 词：附子 炮制 胆巴 氯化钙 生物碱 Box-Behnken响应面法 

分 类 号：R282.71[医药卫生—中药学]