基于网络药理学研究补阳还五汤治疗急性高原低氧性脑损伤的作用机制  

Study of the mechanism of Buyang Huanwu decoction in treating acute high-altitude hypoxic brain injury based on network pharmacology

作　　者：黎洁 李永平[1] 朱港星 LI Jie;LI Yongping;ZHU Gangxing(Qinghai Provincial Key Laboratory of Traditional Chinese Medicine Research for Glucolipid Metabolic Diseases,Qinghai University,Xining 810001,China)

机构地区：[1]青海大学医学部青海省糖脂代谢疾病防控中医药重点实验室,西宁810001

出　　处：《环球中医药》2023年第5期889-897,共9页Global Traditional Chinese Medicine

基　　金：青海省科技厅应用基础研究项目(2020-ZJ-760);青海省中藏医药科研课题项目(J2023003)。

摘　　要：目的基于网络药理学研究补阳还五汤治疗急性高原低氧性脑损伤的有效活性成分及作用靶点,分析其作用机制。方法运用TCMSP、Swiss ADME、Swiss Target Prediction数据库筛选补阳还五汤的有效成分及作用靶点;运用OMIM、Gene Cards数据库获取急性高原低氧性脑损伤的疾病靶点,筛出疾病与药物的共同靶点;运用String在线数据库构建疾病与药物蛋白质相互作用图;运用Cytoscape3.7.1软件绘制“药物-活性成分-靶点-疾病”网络图;运用Metascape在线数据库进行GO分析和KEGG信号通路富集分析。结果补阳还五汤中发挥治疗急性高原低氧性脑损伤的主要成分是木犀草素、杨梅酮、芒柄花黄素、山奈酚、鞣花酸、毛蕊异黄酮等活性成分,主要靶点是蛋白激酶B1、白介素-6、肿瘤坏死因子、肿瘤蛋白P53、血管内皮生长因子A、白介素-1β、缺氧诱导因子1α等;通路主要富集在磷脂酰肌醇-3-激酶/蛋白激酶B信号通路、缺氧诱导因子-1信号通路、Janus激酶/信号转导子和转录激活子信号通路等。结论补阳还五汤治疗急性高原低氧性脑损伤的机制可能是通过调节炎症反应、抗氧化应激、减少细胞凋亡等协同作用保护神经元细胞,可为临床应用提供一定的参考依据。Objective Based on network pharmacology,the active ingredient and target of Buyang Huanwu decoction in the treatment of acute high-altitude hypoxic brain injury were studied,and its mechanism of action was analyzed.Methods TCMSP,Swiss ADME and Swiss Target Prediction databases were used to screen the active ingredients and targets of Buyang Huanwu decoction;OMIM,Gene Cards,drug bank databases were used to obtain disease targets of acute high-altitude hypoxic brain injury,and common targets of diseases and drugs were screened out.The network diagram of disease-drug protein interaction(PPI)was constructed by using the String online database.Cytoscape3.7.1 software was used to draw a network map of“drug-active ingredient-target-disease”;GO analysis and KEGG signal pathway enrichment were analyzed by using Metascape online database.Results The main ingredients in the treatment of acute high-altitude hypoxic brain injury in the Buyang Huanwu decoction were rosolin,baymethone,mangosteendoxanthin,kaempferol,ellagic acid,mullein isoflavones and other active ingredients,the main targets were Akt1,IL-6,TNF,TP53,VEGFA,IL-1β,HIF1αway;the channels were mainly enriched in PI3K/Akt signal pathway,HIF-1 signal path,JAK/STAT signal pathway and so on.Conclusion It is revealed that the mechanism of the treatment of acute high-altitude hypoxic brain injury may be to protect neuronal cells by regulating the synergistic effects of inflammatory response,antioxidant stress,and reducing apoptosis,which can provide a certain reference for clinical applications.

关 键 词：补阳还五汤 急性高原低氧性脑损伤 炎症反应 抗氧化应激 细胞凋亡 

分 类 号：R649.9[医药卫生—外科学]